Escherichia coli O157:H7 strains isolated from high-event period beef contamination have strong biofilm-forming ability and low sanitizer susceptibility, which are associated with high pO157 plasmid copy number

Rong Wang, Brandon E. Luedtke, Joseph M. Bosilevac, John W. Schmidt, Norasak Kalchayanand, Terrance M. Arthur

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the meat industry, a high-event period (HEP) is defined as a time period when beef processing establishments experience an increased occurrence of product contamination by Escherichia coli O157:H7. Our previous studies suggested that bacterial biofilm formation and sanitizer resistance might contribute to HEPs. We conducted the present study to further characterize E. coli O157:H7 strains isolated during HEPs for their potential to cause contamination and to investigate the genetic basis for their strong biofilm-forming ability and high sanitizer resistance. Our results show that, compared with the E. coli O157:H7 diversity control panel strains, the HEP strains had a significantly higher biofilm-forming ability on contact surfaces and a lower susceptibility to common sanitizers. No difference in the presence of disinfectant-resistant genes or the prevalence of antibiotic resistance was observed between the HEP and control strains. However, the HEP strains retained significantly higher copy numbers of the pO157 plasmid. A positive correlation was observed among a strain's high plasmid copy number, strong biofilm-forming ability, low sanitizer susceptibility, and high survival and recovery capability after sanitization, suggesting that these specific phenotypes could be either directly correlated to gene expression on the pO157 plasmid or indirectly regulated via chromosomal gene expression influenced by the presence of the plasmid. Our data highlight the potential risk of biofilm formation and sanitizer resistance in HEP contamination by E. coli O157:H7, and our results call for increased attention to proper and effective sanitization practices in meat processing facilities.

Original languageEnglish (US)
Pages (from-to)1875-1883
Number of pages9
JournalJournal of food protection
Volume79
Issue number11
DOIs
StatePublished - Nov 2016

Fingerprint

sanitizers
Escherichia coli O157
Biofilms
biofilm
plasmids
beef
Plasmids
Meat
Gene Expression
Disinfectants
meat processing
gene expression
Microbial Drug Resistance
livestock and meat industry
disinfectants
Industry
antibiotic resistance
Red Meat
Phenotype
phenotype

Keywords

  • Biofilm
  • Escherichia coli O157:H7
  • High-event period
  • Sanitizer
  • pO157 plasmid

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Escherichia coli O157:H7 strains isolated from high-event period beef contamination have strong biofilm-forming ability and low sanitizer susceptibility, which are associated with high pO157 plasmid copy number. / Wang, Rong; Luedtke, Brandon E.; Bosilevac, Joseph M.; Schmidt, John W.; Kalchayanand, Norasak; Arthur, Terrance M.

In: Journal of food protection, Vol. 79, No. 11, 11.2016, p. 1875-1883.

Research output: Contribution to journalArticle

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abstract = "In the meat industry, a high-event period (HEP) is defined as a time period when beef processing establishments experience an increased occurrence of product contamination by Escherichia coli O157:H7. Our previous studies suggested that bacterial biofilm formation and sanitizer resistance might contribute to HEPs. We conducted the present study to further characterize E. coli O157:H7 strains isolated during HEPs for their potential to cause contamination and to investigate the genetic basis for their strong biofilm-forming ability and high sanitizer resistance. Our results show that, compared with the E. coli O157:H7 diversity control panel strains, the HEP strains had a significantly higher biofilm-forming ability on contact surfaces and a lower susceptibility to common sanitizers. No difference in the presence of disinfectant-resistant genes or the prevalence of antibiotic resistance was observed between the HEP and control strains. However, the HEP strains retained significantly higher copy numbers of the pO157 plasmid. A positive correlation was observed among a strain's high plasmid copy number, strong biofilm-forming ability, low sanitizer susceptibility, and high survival and recovery capability after sanitization, suggesting that these specific phenotypes could be either directly correlated to gene expression on the pO157 plasmid or indirectly regulated via chromosomal gene expression influenced by the presence of the plasmid. Our data highlight the potential risk of biofilm formation and sanitizer resistance in HEP contamination by E. coli O157:H7, and our results call for increased attention to proper and effective sanitization practices in meat processing facilities.",
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