Escherichia coli O157:H7 in a cohort of weaned, preconditioned range beef calves

John R. Dunn, James E. Keen, Ron Del Vecchio, Thomas E. Wittum, R. Alex Thompson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Escherichia coli O157:H7 (EC O157) is an important cause of foodborne disease. Cattle are reservoirs for the bacteria and are implicated in transmission to humans. Prevalence data in prefeedlot calves are limited. With the use of sensitive methods, a cohort of weaned beef calves (n = 408) was sampled before and after preconditioning to estimate fecal point prevalence and describe changes in EC O157 fecal shedding. EC O157 isolates were confirmed and characterized by PCR and pulsed-field gel electrophoresis. Calves from 29 cow-calf farms were commingled at three preconditioning sites and placed on a transition ration containing oxytetracycline (200 g/ton) for 45 days. Initial animal-level fecal point prevalence was 2.5% (95% confidence interval, 1 to 5) with a herd-level prevalence of 17.2% (95% confidence interval, 6 to 36). Point prevalence following the preconditioning feeding period was 0%. An unexpected finding in our study was EC O157 isolates that were Shiga toxin-deficient. Pulsed-field gel electrophoresis subtypes of EC O157 were unique in epidemiologically unlinked herds, except one herd that had two unique subtypes. We expected, but observed, neither increased fecal shedding in the cohort nor horizontal transmission of unique EC O157 subtypes. The absence of fecal shedding following the 45-day feeding period might be attributable to seasonal influences, inhibitory concentrations of oxytetracycline in the transition ration, or transient colonization that ended before sampling. EC O157 is apparently widely dispersed at low prevalence in U.S. prefeedlot, weaned calves.

Original languageEnglish (US)
Pages (from-to)2391-2396
Number of pages6
JournalJournal of food protection
Volume67
Issue number11
DOIs
StatePublished - Nov 2004

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Escherichia coli O157
beef
calves
Oxytetracycline
Pulsed Field Gel Electrophoresis
herds
oxytetracycline
pulsed-field gel electrophoresis
confidence interval
Confidence Intervals
Shiga Toxin
Shiga toxin
Foodborne Diseases
foodborne illness
Red Meat
Bacteria
cows
Polymerase Chain Reaction
farms
cattle

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Escherichia coli O157:H7 in a cohort of weaned, preconditioned range beef calves. / Dunn, John R.; Keen, James E.; Del Vecchio, Ron; Wittum, Thomas E.; Thompson, R. Alex.

In: Journal of food protection, Vol. 67, No. 11, 11.2004, p. 2391-2396.

Research output: Contribution to journalArticle

Dunn, John R. ; Keen, James E. ; Del Vecchio, Ron ; Wittum, Thomas E. ; Thompson, R. Alex. / Escherichia coli O157:H7 in a cohort of weaned, preconditioned range beef calves. In: Journal of food protection. 2004 ; Vol. 67, No. 11. pp. 2391-2396.
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