Erbin controls dendritic morphogenesis by regulating localization of δ-catenin

Jyothi Arikkath, Inbal Israely, Yanmei Tao, Lin Mei, Xin Liu, Louis F. Reichardt

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The LAP [leucine-rich and postsynaptic density-95/Discs large/zona occludens-1 (PDZ)] protein erbin and δ-catenin, a component of the cadherin- catenin cell adhesion complex, are highly expressed in neurons and associate through PDZ-mediated interaction, but have incompletely characterized neuronal functions. We show that short hairpin RNA-mediated knockdown of erbin and knockdown or genetic ablation of δ-catenin severely impaired dendritic morphogenesis in hippocampal neurons. Simultaneous loss of erbin and δ-catenin does not enhance severity of this phenotype. The dendritic phenotype observed after erbin depletion is rescued by overexpression of δ-catenin and requires a domain in δ-catenin that has been shown to regulate dendritic branching. Knockdown of δ-catenin cannot be rescued by overexpression of erbin, indicating that erbin is upstream of δ-catenin. δ-Catenin-null neurons have no alterations in global levels of active Rac1/RhoA. Knockdown of erbin results in alterations in localization of δ-catenin. These results suggest a critical role for erbin in regulating dendritic morphogenesis by maintaining appropriate localization of δ-catenin.

Original languageEnglish (US)
Pages (from-to)7047-7056
Number of pages10
JournalJournal of Neuroscience
Volume28
Issue number28
DOIs
StatePublished - Jul 9 2008

Fingerprint

Catenins
Morphogenesis
Neurons
Zonula Occludens-1 Protein
Phenotype
Post-Synaptic Density
Cadherins
Cell Adhesion
Leucine
Small Interfering RNA

Keywords

  • Dendrite
  • Erbin
  • LAP proteins
  • Localization
  • PDZ
  • δ-catenin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Erbin controls dendritic morphogenesis by regulating localization of δ-catenin. / Arikkath, Jyothi; Israely, Inbal; Tao, Yanmei; Mei, Lin; Liu, Xin; Reichardt, Louis F.

In: Journal of Neuroscience, Vol. 28, No. 28, 09.07.2008, p. 7047-7056.

Research output: Contribution to journalArticle

Arikkath, Jyothi ; Israely, Inbal ; Tao, Yanmei ; Mei, Lin ; Liu, Xin ; Reichardt, Louis F. / Erbin controls dendritic morphogenesis by regulating localization of δ-catenin. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 28. pp. 7047-7056.
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