Ept7 influences estrogen action in the pituitary gland and body weight of rats

Scott G. Kurz, Kirsten L. Dennison, Nyssa Becker Samanas, Maureen Peters Hickman, Quincy A. Eckert, Tiffany L. Walker, Andrea S Cupp, James D. Shull

Research output: Contribution to journalArticle

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Abstract

Estrogens control many aspects of pituitary gland biology, including regulation of lactotroph homeostasis and synthesis and secretion of prolactin. In rat models, these actions are strain specific and heritable, and multiple quantitative trait loci (QTL) have been mapped that impact the responsiveness of the lactotroph to estrogens. One such QTL, Ept7, was mapped to RNO7 in female progeny generated in an intercross between BN rats, in which the lactotroph population is insensitive to estrogens, and ACI rats, which develop lactotroph hyperplasia/adenoma and associated hyperprolactinemia in response to estrogen treatment. The primary objective of this study was to confirm the existence of Ept7 and to quantify the impact of this QTL on responsiveness of the pituitary gland of female and male rats to 17β-estradiol (E2) and diethylstilbestrol (DES), respectively. Secondary objectives were to determine if Ept7 influences the responsiveness of the male reproductive tract to DES and to identify other discernible phenotypes influenced by Ept7. To achieve these objectives, a congenic rat strain that harbors BN alleles across the Ept7 interval on the genetic background of the ACI strain was generated and characterized to define the effect of administered estrogens on the anterior pituitary gland and male reproductive tissues. Data presented herein indicate Ept7 exerts a marked effect on development of lactotroph hyperplasia in response to estrogen treatment, but does not affect atrophy of the male reproductive tissues in response to hormone treatment. Ept7 was also observed to exert gender specific effects on body weight in young adult rats.

Original languageEnglish (US)
Pages (from-to)244-252
Number of pages9
JournalMammalian Genome
Volume25
Issue number5-6
DOIs
StatePublished - Jan 1 2014

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Pituitary Gland
Lactotrophs
Estrogens
Body Weight
Quantitative Trait Loci
Diethylstilbestrol
Heritable Quantitative Trait
Hyperplasia
Inbred BN Rats
Inbred ACI Rats
Prolactinoma
Hyperprolactinemia
Anterior Pituitary Gland
Prolactin
Atrophy
Young Adult
Estradiol
Homeostasis
Therapeutics
Alleles

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Kurz, S. G., Dennison, K. L., Samanas, N. B., Hickman, M. P., Eckert, Q. A., Walker, T. L., ... Shull, J. D. (2014). Ept7 influences estrogen action in the pituitary gland and body weight of rats. Mammalian Genome, 25(5-6), 244-252. https://doi.org/10.1007/s00335-014-9504-4

Ept7 influences estrogen action in the pituitary gland and body weight of rats. / Kurz, Scott G.; Dennison, Kirsten L.; Samanas, Nyssa Becker; Hickman, Maureen Peters; Eckert, Quincy A.; Walker, Tiffany L.; Cupp, Andrea S; Shull, James D.

In: Mammalian Genome, Vol. 25, No. 5-6, 01.01.2014, p. 244-252.

Research output: Contribution to journalArticle

Kurz, SG, Dennison, KL, Samanas, NB, Hickman, MP, Eckert, QA, Walker, TL, Cupp, AS & Shull, JD 2014, 'Ept7 influences estrogen action in the pituitary gland and body weight of rats', Mammalian Genome, vol. 25, no. 5-6, pp. 244-252. https://doi.org/10.1007/s00335-014-9504-4
Kurz SG, Dennison KL, Samanas NB, Hickman MP, Eckert QA, Walker TL et al. Ept7 influences estrogen action in the pituitary gland and body weight of rats. Mammalian Genome. 2014 Jan 1;25(5-6):244-252. https://doi.org/10.1007/s00335-014-9504-4
Kurz, Scott G. ; Dennison, Kirsten L. ; Samanas, Nyssa Becker ; Hickman, Maureen Peters ; Eckert, Quincy A. ; Walker, Tiffany L. ; Cupp, Andrea S ; Shull, James D. / Ept7 influences estrogen action in the pituitary gland and body weight of rats. In: Mammalian Genome. 2014 ; Vol. 25, No. 5-6. pp. 244-252.
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