Abstract
Fifty-five consecutive cases of Hodgkin's lymphoma (HL), collected between 1996 and 1998 from Cairo, Egypt, were histologically subtyped, phenotyped, and then studied for the presence of Epstein-Barr virus (EBV). We used immunohistochemical stains for EBV latent membrane protein 1 (LMP-1) and in situ hybridization stains for EBV-encoded small RNA (EBER-1) transcripts. Forty-five cases (82%) had classic HL (cHL), and ten cases (18%) had nodular lymphocyte predominant HL (NLPHL), with each group expressing its typical phenotype. LMP-1 stains were positive in 63% and 0% of cHL and NLPHL cases, respectively. EBER-positive Reed-Sternberg cells and variants were also present in 62% and 0% of each group, respectively. The cHL cases showed variable EBER positivity: nodular sclerosis, 58%; mixed cellularity, 100%; lymphocyte depletion, 100%; and unclassifiable, 67%. Our findings are similar to those from other developing countries and point towards a pathogenic role of EBV in cHL.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-18 |
| Number of pages | 8 |
| Journal | Journal of Hematopathology |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1 2010 |
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Keywords
- Classic Hodgkin's lymphoma
- EBER-1
- Epstein-Barr virus
- LMP-1
- Nodular lymphocyte predominant Hodgkin's lymphoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
- Hematology
Cite this
Epstein-Barr virus and Hodgkin's lymphoma in Cairo, Egypt. / Audouin, Josée; Diebold, Jacques; Nathwani, Bharat; Ishak, Elia; MacLennan, Kenneth; Mueller-Hermelink, Hans Konrad; Armitage, James Olen; Weisenburger, Dennis D.
In: Journal of Hematopathology, Vol. 3, No. 1, 01.06.2010, p. 11-18.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epstein-Barr virus and Hodgkin's lymphoma in Cairo, Egypt
AU - Audouin, Josée
AU - Diebold, Jacques
AU - Nathwani, Bharat
AU - Ishak, Elia
AU - MacLennan, Kenneth
AU - Mueller-Hermelink, Hans Konrad
AU - Armitage, James Olen
AU - Weisenburger, Dennis D.
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Fifty-five consecutive cases of Hodgkin's lymphoma (HL), collected between 1996 and 1998 from Cairo, Egypt, were histologically subtyped, phenotyped, and then studied for the presence of Epstein-Barr virus (EBV). We used immunohistochemical stains for EBV latent membrane protein 1 (LMP-1) and in situ hybridization stains for EBV-encoded small RNA (EBER-1) transcripts. Forty-five cases (82%) had classic HL (cHL), and ten cases (18%) had nodular lymphocyte predominant HL (NLPHL), with each group expressing its typical phenotype. LMP-1 stains were positive in 63% and 0% of cHL and NLPHL cases, respectively. EBER-positive Reed-Sternberg cells and variants were also present in 62% and 0% of each group, respectively. The cHL cases showed variable EBER positivity: nodular sclerosis, 58%; mixed cellularity, 100%; lymphocyte depletion, 100%; and unclassifiable, 67%. Our findings are similar to those from other developing countries and point towards a pathogenic role of EBV in cHL.
AB - Fifty-five consecutive cases of Hodgkin's lymphoma (HL), collected between 1996 and 1998 from Cairo, Egypt, were histologically subtyped, phenotyped, and then studied for the presence of Epstein-Barr virus (EBV). We used immunohistochemical stains for EBV latent membrane protein 1 (LMP-1) and in situ hybridization stains for EBV-encoded small RNA (EBER-1) transcripts. Forty-five cases (82%) had classic HL (cHL), and ten cases (18%) had nodular lymphocyte predominant HL (NLPHL), with each group expressing its typical phenotype. LMP-1 stains were positive in 63% and 0% of cHL and NLPHL cases, respectively. EBER-positive Reed-Sternberg cells and variants were also present in 62% and 0% of each group, respectively. The cHL cases showed variable EBER positivity: nodular sclerosis, 58%; mixed cellularity, 100%; lymphocyte depletion, 100%; and unclassifiable, 67%. Our findings are similar to those from other developing countries and point towards a pathogenic role of EBV in cHL.
KW - Classic Hodgkin's lymphoma
KW - EBER-1
KW - Epstein-Barr virus
KW - LMP-1
KW - Nodular lymphocyte predominant Hodgkin's lymphoma
UR - http://www.scopus.com/inward/record.url?scp=84873860827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873860827&partnerID=8YFLogxK
U2 - 10.1007/s12308-010-0059-3
DO - 10.1007/s12308-010-0059-3
M3 - Article
VL - 3
SP - 11
EP - 18
JO - Journal of Hematopathology
JF - Journal of Hematopathology
SN - 1865-5785
IS - 1
ER -