Epithelial activation in nocturnal asthma

M. Kraft, I. Striz, Stephen Israel Rennard, J. Pak, C. M. Bettinger, M. Rex, R. J. Martin

Research output: Contribution to journalArticle

Abstract

Our laboratory has shown greater alveolar tissue inflammation in nocturnal asthmatics (NA) at night as compared to the large airways. We hypothesized that epithelial surface markers will also be increased in the distal airways of patients with NA at night. In an ongoing study, we evaluated nine patients, four with NA and five with non-nocturnal asthma (NNA) at 4 am and 4 pm. All patients underwent bronchoscopy at both time points with endobronchial brushing of the proximal airways under direct visualization and the distal airways under fluoroscopic guidance using a cytological brush. Epithelial cells were stained for: CD54 (ICAM-1), HLA-DR (activation marker), CD1lb (CR3 receptor), CD29 (β-1 integrin), CD51 (vitronectin receptor) and CD49b (VLA-2, collagen receptor). The % predicted FEV1 was significantly reduced in the NA group as compared to the NNA group at 4 am (60.3 ± 2.1% vs. 81.6 ± 2.4%, p=0.001). The % of cells staining for CD51 and CD49b were increased in the NA group as compared to the NNA group, but only in the distal tissue (CD51: 43.0 ± 5.1% vs. 21.2 ± 4.6%, p=0.02; CD49b: 32.0 ± 4.1% vs. 15.4 ± 3.7%, p= 0.02). In the NA group alone, CD29 and CD51 were greater in the distal tissue at 4 am as compared to 4 pm (CD29; 88.0 ± 3.9% vs. 77.0 ± 6.5%, p=0.024; CD51: 43.0 ± 5.1% vs. 24.0 ± 3.5%, p=0.025). A significant relationship was present between the % predicted 4 am FEV1 and the % of CD49b positive cells in the distal airways (r=0.74,p=0.05). There were no differences in the numbers of cells staining for any of the markers in the proximal airways at either time point. These preliminary findings suggest that markers of epithelial activation are increased at night in the distal airways of patients with NA.

Original languageEnglish (US)
JournalJournal of Investigative Medicine
Volume44
Issue number1
StatePublished - Jan 1 1996

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Asthma
Chemical activation
Integrin alpha2beta1
Integrin alphaVbeta3
Macrophage-1 Antigen
Collagen Receptors
Staining and Labeling
Tissue
Bronchoscopy
HLA-DR Antigens
Intercellular Adhesion Molecule-1
Integrins
Cell Count
Epithelial Cells
Inflammation
Brushes
Visualization
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kraft, M., Striz, I., Rennard, S. I., Pak, J., Bettinger, C. M., Rex, M., & Martin, R. J. (1996). Epithelial activation in nocturnal asthma. Journal of Investigative Medicine, 44(1).

Epithelial activation in nocturnal asthma. / Kraft, M.; Striz, I.; Rennard, Stephen Israel; Pak, J.; Bettinger, C. M.; Rex, M.; Martin, R. J.

In: Journal of Investigative Medicine, Vol. 44, No. 1, 01.01.1996.

Research output: Contribution to journalArticle

Kraft, M, Striz, I, Rennard, SI, Pak, J, Bettinger, CM, Rex, M & Martin, RJ 1996, 'Epithelial activation in nocturnal asthma', Journal of Investigative Medicine, vol. 44, no. 1.
Kraft M, Striz I, Rennard SI, Pak J, Bettinger CM, Rex M et al. Epithelial activation in nocturnal asthma. Journal of Investigative Medicine. 1996 Jan 1;44(1).
Kraft, M. ; Striz, I. ; Rennard, Stephen Israel ; Pak, J. ; Bettinger, C. M. ; Rex, M. ; Martin, R. J. / Epithelial activation in nocturnal asthma. In: Journal of Investigative Medicine. 1996 ; Vol. 44, No. 1.
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abstract = "Our laboratory has shown greater alveolar tissue inflammation in nocturnal asthmatics (NA) at night as compared to the large airways. We hypothesized that epithelial surface markers will also be increased in the distal airways of patients with NA at night. In an ongoing study, we evaluated nine patients, four with NA and five with non-nocturnal asthma (NNA) at 4 am and 4 pm. All patients underwent bronchoscopy at both time points with endobronchial brushing of the proximal airways under direct visualization and the distal airways under fluoroscopic guidance using a cytological brush. Epithelial cells were stained for: CD54 (ICAM-1), HLA-DR (activation marker), CD1lb (CR3 receptor), CD29 (β-1 integrin), CD51 (vitronectin receptor) and CD49b (VLA-2, collagen receptor). The {\%} predicted FEV1 was significantly reduced in the NA group as compared to the NNA group at 4 am (60.3 ± 2.1{\%} vs. 81.6 ± 2.4{\%}, p=0.001). The {\%} of cells staining for CD51 and CD49b were increased in the NA group as compared to the NNA group, but only in the distal tissue (CD51: 43.0 ± 5.1{\%} vs. 21.2 ± 4.6{\%}, p=0.02; CD49b: 32.0 ± 4.1{\%} vs. 15.4 ± 3.7{\%}, p= 0.02). In the NA group alone, CD29 and CD51 were greater in the distal tissue at 4 am as compared to 4 pm (CD29; 88.0 ± 3.9{\%} vs. 77.0 ± 6.5{\%}, p=0.024; CD51: 43.0 ± 5.1{\%} vs. 24.0 ± 3.5{\%}, p=0.025). A significant relationship was present between the {\%} predicted 4 am FEV1 and the {\%} of CD49b positive cells in the distal airways (r=0.74,p=0.05). There were no differences in the numbers of cells staining for any of the markers in the proximal airways at either time point. These preliminary findings suggest that markers of epithelial activation are increased at night in the distal airways of patients with NA.",
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AU - Rex, M.

AU - Martin, R. J.

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N2 - Our laboratory has shown greater alveolar tissue inflammation in nocturnal asthmatics (NA) at night as compared to the large airways. We hypothesized that epithelial surface markers will also be increased in the distal airways of patients with NA at night. In an ongoing study, we evaluated nine patients, four with NA and five with non-nocturnal asthma (NNA) at 4 am and 4 pm. All patients underwent bronchoscopy at both time points with endobronchial brushing of the proximal airways under direct visualization and the distal airways under fluoroscopic guidance using a cytological brush. Epithelial cells were stained for: CD54 (ICAM-1), HLA-DR (activation marker), CD1lb (CR3 receptor), CD29 (β-1 integrin), CD51 (vitronectin receptor) and CD49b (VLA-2, collagen receptor). The % predicted FEV1 was significantly reduced in the NA group as compared to the NNA group at 4 am (60.3 ± 2.1% vs. 81.6 ± 2.4%, p=0.001). The % of cells staining for CD51 and CD49b were increased in the NA group as compared to the NNA group, but only in the distal tissue (CD51: 43.0 ± 5.1% vs. 21.2 ± 4.6%, p=0.02; CD49b: 32.0 ± 4.1% vs. 15.4 ± 3.7%, p= 0.02). In the NA group alone, CD29 and CD51 were greater in the distal tissue at 4 am as compared to 4 pm (CD29; 88.0 ± 3.9% vs. 77.0 ± 6.5%, p=0.024; CD51: 43.0 ± 5.1% vs. 24.0 ± 3.5%, p=0.025). A significant relationship was present between the % predicted 4 am FEV1 and the % of CD49b positive cells in the distal airways (r=0.74,p=0.05). There were no differences in the numbers of cells staining for any of the markers in the proximal airways at either time point. These preliminary findings suggest that markers of epithelial activation are increased at night in the distal airways of patients with NA.

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