Epidermal growth factor and transforming growth factor-β modulation of follicle-stimulating hormone-induced deoxyribonucleic acid synthesis in hamster preantral and early antral follicles

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The interactions of epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) in modulating FSH-induced follicular DNA synthesis were studied in isolated intact preantral (stages 1-6) and early antral (stage 7) ovarian follicles from adult hamsters. Follicles were exposed in vitro for 24 h to FSH (100 ng), EGF (50 ng), TGF-β1 (1-10 ng), or TGF-β2 (1-10 ng), either alone or in combination. The rate of DNA synthesis was assessed by measuring the incorporation of [3H]thymidine into follicular DNA. Both EGF and FSH significantly stimulated follicular DNA synthesis compared with that in controls. Both isoforms of TGF-β significantly increased follicular [3H]thymidine incorporation in a dose-dependent manner; the effect was greater for small preantral follicles, such as those of stages 1-4. Interestingly, TGF-β significantly inhibited EGF-induced follicular DNA synthesis, but the rates of DNA synthesis for most of the stages were still higher than that of the control follicles. Specificity of the TGF-β action on follicular DNA synthesis was evident from the ability of isoform-specific anti-TGF-β antibodies to neutralize the effect. These antibodies also reversed the TGF-β inhibition of EGF-induced DNA synthesis. Surprisingly, although TGF-β attenuated EGF-induced DNA synthesis, it synergized with FSH to stimulate follicular DNA synthesis. Interestingly, FSH-induced DNA synthesis remained unaffected by the anti-TGF-β antibodies, indicating that TGF-β may not mediate FSH action on follicular DNA synthesis. These studies suggest that a critical interaction of EGF and TGF-β modulates granulosa cell proliferation during folliculogenesis in the hamster. TGF-β may also influence FSH regulation of cell proliferation by lengthening the G1 phase of the granulosa cell cycle, thus recruiting more cells to enter the S phase when the preovulatory FSH surge acts on growing follicles.

Original languageEnglish (US)
Pages (from-to)552-557
Number of pages6
JournalBiology of reproduction
Volume48
Issue number3
DOIs
StatePublished - Mar 1 1993

Fingerprint

Follicle Stimulating Hormone
Transforming Growth Factors
Epidermal Growth Factor
Cricetinae
DNA
Granulosa Cells
Antral
Thymidine
Antibodies
Protein Isoforms
Cell Proliferation
Gonadotrophs
Ovarian Follicle
G1 Phase
S Phase
Cell Cycle

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

@article{b65e25c853344af283b813bac7e373df,
title = "Epidermal growth factor and transforming growth factor-β modulation of follicle-stimulating hormone-induced deoxyribonucleic acid synthesis in hamster preantral and early antral follicles",
abstract = "The interactions of epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) in modulating FSH-induced follicular DNA synthesis were studied in isolated intact preantral (stages 1-6) and early antral (stage 7) ovarian follicles from adult hamsters. Follicles were exposed in vitro for 24 h to FSH (100 ng), EGF (50 ng), TGF-β1 (1-10 ng), or TGF-β2 (1-10 ng), either alone or in combination. The rate of DNA synthesis was assessed by measuring the incorporation of [3H]thymidine into follicular DNA. Both EGF and FSH significantly stimulated follicular DNA synthesis compared with that in controls. Both isoforms of TGF-β significantly increased follicular [3H]thymidine incorporation in a dose-dependent manner; the effect was greater for small preantral follicles, such as those of stages 1-4. Interestingly, TGF-β significantly inhibited EGF-induced follicular DNA synthesis, but the rates of DNA synthesis for most of the stages were still higher than that of the control follicles. Specificity of the TGF-β action on follicular DNA synthesis was evident from the ability of isoform-specific anti-TGF-β antibodies to neutralize the effect. These antibodies also reversed the TGF-β inhibition of EGF-induced DNA synthesis. Surprisingly, although TGF-β attenuated EGF-induced DNA synthesis, it synergized with FSH to stimulate follicular DNA synthesis. Interestingly, FSH-induced DNA synthesis remained unaffected by the anti-TGF-β antibodies, indicating that TGF-β may not mediate FSH action on follicular DNA synthesis. These studies suggest that a critical interaction of EGF and TGF-β modulates granulosa cell proliferation during folliculogenesis in the hamster. TGF-β may also influence FSH regulation of cell proliferation by lengthening the G1 phase of the granulosa cell cycle, thus recruiting more cells to enter the S phase when the preovulatory FSH surge acts on growing follicles.",
author = "Roy, {Shyamal K}",
year = "1993",
month = "3",
day = "1",
doi = "10.1095/biolreprod48.3.552",
language = "English (US)",
volume = "48",
pages = "552--557",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "3",

}

TY - JOUR

T1 - Epidermal growth factor and transforming growth factor-β modulation of follicle-stimulating hormone-induced deoxyribonucleic acid synthesis in hamster preantral and early antral follicles

AU - Roy, Shyamal K

PY - 1993/3/1

Y1 - 1993/3/1

N2 - The interactions of epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) in modulating FSH-induced follicular DNA synthesis were studied in isolated intact preantral (stages 1-6) and early antral (stage 7) ovarian follicles from adult hamsters. Follicles were exposed in vitro for 24 h to FSH (100 ng), EGF (50 ng), TGF-β1 (1-10 ng), or TGF-β2 (1-10 ng), either alone or in combination. The rate of DNA synthesis was assessed by measuring the incorporation of [3H]thymidine into follicular DNA. Both EGF and FSH significantly stimulated follicular DNA synthesis compared with that in controls. Both isoforms of TGF-β significantly increased follicular [3H]thymidine incorporation in a dose-dependent manner; the effect was greater for small preantral follicles, such as those of stages 1-4. Interestingly, TGF-β significantly inhibited EGF-induced follicular DNA synthesis, but the rates of DNA synthesis for most of the stages were still higher than that of the control follicles. Specificity of the TGF-β action on follicular DNA synthesis was evident from the ability of isoform-specific anti-TGF-β antibodies to neutralize the effect. These antibodies also reversed the TGF-β inhibition of EGF-induced DNA synthesis. Surprisingly, although TGF-β attenuated EGF-induced DNA synthesis, it synergized with FSH to stimulate follicular DNA synthesis. Interestingly, FSH-induced DNA synthesis remained unaffected by the anti-TGF-β antibodies, indicating that TGF-β may not mediate FSH action on follicular DNA synthesis. These studies suggest that a critical interaction of EGF and TGF-β modulates granulosa cell proliferation during folliculogenesis in the hamster. TGF-β may also influence FSH regulation of cell proliferation by lengthening the G1 phase of the granulosa cell cycle, thus recruiting more cells to enter the S phase when the preovulatory FSH surge acts on growing follicles.

AB - The interactions of epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) in modulating FSH-induced follicular DNA synthesis were studied in isolated intact preantral (stages 1-6) and early antral (stage 7) ovarian follicles from adult hamsters. Follicles were exposed in vitro for 24 h to FSH (100 ng), EGF (50 ng), TGF-β1 (1-10 ng), or TGF-β2 (1-10 ng), either alone or in combination. The rate of DNA synthesis was assessed by measuring the incorporation of [3H]thymidine into follicular DNA. Both EGF and FSH significantly stimulated follicular DNA synthesis compared with that in controls. Both isoforms of TGF-β significantly increased follicular [3H]thymidine incorporation in a dose-dependent manner; the effect was greater for small preantral follicles, such as those of stages 1-4. Interestingly, TGF-β significantly inhibited EGF-induced follicular DNA synthesis, but the rates of DNA synthesis for most of the stages were still higher than that of the control follicles. Specificity of the TGF-β action on follicular DNA synthesis was evident from the ability of isoform-specific anti-TGF-β antibodies to neutralize the effect. These antibodies also reversed the TGF-β inhibition of EGF-induced DNA synthesis. Surprisingly, although TGF-β attenuated EGF-induced DNA synthesis, it synergized with FSH to stimulate follicular DNA synthesis. Interestingly, FSH-induced DNA synthesis remained unaffected by the anti-TGF-β antibodies, indicating that TGF-β may not mediate FSH action on follicular DNA synthesis. These studies suggest that a critical interaction of EGF and TGF-β modulates granulosa cell proliferation during folliculogenesis in the hamster. TGF-β may also influence FSH regulation of cell proliferation by lengthening the G1 phase of the granulosa cell cycle, thus recruiting more cells to enter the S phase when the preovulatory FSH surge acts on growing follicles.

UR - http://www.scopus.com/inward/record.url?scp=0027525991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027525991&partnerID=8YFLogxK

U2 - 10.1095/biolreprod48.3.552

DO - 10.1095/biolreprod48.3.552

M3 - Article

VL - 48

SP - 552

EP - 557

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 3

ER -