Enzymes of adenosine metabolism in the brain

Diumal rhythm and the effect of sleep deprivation

Miroslaw Mackiewicz, Elena V. Nikonova, John E. Zimmerman, Raymond J. Galante, Lin Zhang, Jacqueline R. Cater, Jonathan Geiger, Allan I. Pack

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Adenosine plays a role in promoting sleep, an effect that is thought to be mediated in the basal forebrain. Adenosine levels vary in this region with prolonged wakefulness in a unique way. The basis for this is unknown. We examined, in rats, the activity of the major metabolic enzymes for adenosine - adenosine deaminase, adenosine kinase, ecto- and cytosolic 5′-nucleotidase - in sleep/wake regulatory regions as well as cerebral cortex, and how the activity varies across the day and with sleep deprivation. There were robust spatial differences for the activity of adenosine deaminase, adenosine kinase, and cytosolic and ecto-5′-nucleotidase. However, the basal forebrain was not different from other sleep/wake regulatory regions apart from the tuberomammillary nucleus. All adenosine metabolic enzymes exhibited diurnal variations in their activity, albeit not in all brain regions. Activity of adenosine deaminase increased during the active period in the ventrolateral pre-optic area but decreased significantly in the basal forebrain. Enzymatic activity of adenosine kinase and cytosolic-5′-nucleotidase was higher during the active period in all brain regions tested. However, the activity of ecto-5′-nucleotidase was augmented during the active period only in the cerebral cortex. This diurnal variation may play a role in the regulation of adenosine in relationship to sleep and wakefulness across the day. In contrast, we found no changes specifically with sleep deprivation in the activity of any enzyme in any brain region. Thus, changes in adenosine with sleep deprivation are not a consequence of alterations in adenosine enzyme activity.

Original languageEnglish (US)
Pages (from-to)348-357
Number of pages10
JournalJournal of Neurochemistry
Volume85
Issue number2
DOIs
StatePublished - Jan 1 2003

Fingerprint

Sleep Deprivation
Metabolism
Adenosine
Brain
5'-Nucleotidase
Adenosine Kinase
Enzymes
Adenosine Deaminase
Sleep
Wakefulness
Nucleic Acid Regulatory Sequences
Cerebral Cortex
Lateral Hypothalamic Area
Enzyme activity
Rats
Optics
Basal Forebrain

Keywords

  • Adenosine
  • Adenosine metabolism
  • Basal fore-brain
  • Enzymes
  • Sleep homeostasis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Mackiewicz, M., Nikonova, E. V., Zimmerman, J. E., Galante, R. J., Zhang, L., Cater, J. R., ... Pack, A. I. (2003). Enzymes of adenosine metabolism in the brain: Diumal rhythm and the effect of sleep deprivation. Journal of Neurochemistry, 85(2), 348-357. https://doi.org/10.1046/j.1471-4159.2003.01687.x

Enzymes of adenosine metabolism in the brain : Diumal rhythm and the effect of sleep deprivation. / Mackiewicz, Miroslaw; Nikonova, Elena V.; Zimmerman, John E.; Galante, Raymond J.; Zhang, Lin; Cater, Jacqueline R.; Geiger, Jonathan; Pack, Allan I.

In: Journal of Neurochemistry, Vol. 85, No. 2, 01.01.2003, p. 348-357.

Research output: Contribution to journalArticle

Mackiewicz, M, Nikonova, EV, Zimmerman, JE, Galante, RJ, Zhang, L, Cater, JR, Geiger, J & Pack, AI 2003, 'Enzymes of adenosine metabolism in the brain: Diumal rhythm and the effect of sleep deprivation', Journal of Neurochemistry, vol. 85, no. 2, pp. 348-357. https://doi.org/10.1046/j.1471-4159.2003.01687.x
Mackiewicz, Miroslaw ; Nikonova, Elena V. ; Zimmerman, John E. ; Galante, Raymond J. ; Zhang, Lin ; Cater, Jacqueline R. ; Geiger, Jonathan ; Pack, Allan I. / Enzymes of adenosine metabolism in the brain : Diumal rhythm and the effect of sleep deprivation. In: Journal of Neurochemistry. 2003 ; Vol. 85, No. 2. pp. 348-357.
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