Enzyme-modified casein fibers and their potential application in drug delivery

Li Cui, Narendra Reddy, Helan Xu, Xuerong Fan, Yiqi Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Enzymatic crosslinking of casein fibers was done using Transglutaminase (TGase) to improve the mechanical properties, particularly the stability in aqueous conditions and make them suitable for controlled drug release application. Crosslinking casein with 5 U/g of TGase in the spinning dope for 60 min at 25 °C increased the tenacity and tensile strain of the fibers from 0.40 g/den and 4.2 % to 0.70 g/den and 23.1 %, respectively. The stability of the fibers in water at different pH levels was considerably improved after the enzymatic crosslinking. The SDS-PAGE electrophoresis confirmed that higher molecular weight proteins were formed in TGase-crosslinked fibers. Thermogravimetric analysis (TGA) showed that TGase treated fibers also had a higher thermal degradation temperature than the non-crosslinked fibers. Crosslinked fibers exhibited delayed and lower rate of drug release from the fibers suggesting their suitability for controlled drug release.

Original languageEnglish (US)
Pages (from-to)900-906
Number of pages7
JournalFibers and Polymers
Volume18
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Casein
Caseins
Drug delivery
Enzymes
Transglutaminases
Fibers
Crosslinking
Pharmaceutical Preparations
Tenacity
Tensile strain
Electrophoresis
Thermogravimetric analysis
Pyrolysis
Molecular weight
Proteins
Mechanical properties
Water

Keywords

  • Casein fibers
  • Controlled drug release
  • Crosslinking
  • Transglutaminase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Polymers and Plastics

Cite this

Enzyme-modified casein fibers and their potential application in drug delivery. / Cui, Li; Reddy, Narendra; Xu, Helan; Fan, Xuerong; Yang, Yiqi.

In: Fibers and Polymers, Vol. 18, No. 5, 01.05.2017, p. 900-906.

Research output: Contribution to journalArticle

Cui, Li ; Reddy, Narendra ; Xu, Helan ; Fan, Xuerong ; Yang, Yiqi. / Enzyme-modified casein fibers and their potential application in drug delivery. In: Fibers and Polymers. 2017 ; Vol. 18, No. 5. pp. 900-906.
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