Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera.

L. D. Nelson, Clayton L Kelling

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantification of serum antibodies to transmissible gastroenteritis virus (TGEV) in swine. Sera from pigs inoculated with cell culture-origin TGEV or gut-origin TGEV were tested for anti-TGEV antibody by ELISA and by serum virus-neutralization test (NT). The ELISA detected antibody 3 days (av) sooner than did the NT when sera from pigs inoculated with cell culture-origin TGEV were tested and 1 day sooner than did the NT when sera from pigs inoculated with gut-origin TGEV were tested. The ELISA appeared to be more sensitive than the NT, since ELISA was more responsive to low-level antibody and ELISA titers exceeded NT titers.

Original languageEnglish (US)
Pages (from-to)1654-1657
Number of pages4
JournalAmerican journal of veterinary research
Volume45
Issue number8
StatePublished - Aug 1 1984

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Transmissible gastroenteritis virus
Neutralization Tests
neutralization tests
Swine
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
swine
antibodies
Antibodies
Serum
cell culture
digestive system
Cell Culture Techniques
Viruses

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera. / Nelson, L. D.; Kelling, Clayton L.

In: American journal of veterinary research, Vol. 45, No. 8, 01.08.1984, p. 1654-1657.

Research output: Contribution to journalArticle

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