Enzyme-linked immunosorbent assay (ELISA) for detecting antibodies in sera of patients with adenovirus infection

Geoffrey Milton Thiele, Motohiko Okano, David T. Purtilo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The 41 distinct antigenic types of adenoviruses (Ads) are responsible for a broad spectrum of diseases in humans. We have developed an enzyme-linked immunosorbent assay (ELISA) using adenovirus (Ad) infected MRC-5 cells for detecting IgG and IgM antibodies to Ads. Using the ELISA, we detected IgG antibodies in 100% ( 20 20) of sera from normal adults (geometric mean titer, GMT = 1840.8, range = 40-20,480) and IgM antibodies in 3 of 20 sera (15%) with a GMT of 25.1. Our indirect immunofluorescence (IF) technique also detected IgG antibodies in 100%) of these sera (GMT = 248.3, range = 40-5,120) and IgM antibodies in the 3 samples reactive in ELISA (GMT = 20.0, range = < 5-40). In contrast, the complement fixation (CF) test detected antibodies to Ads in only 65% ( 13 20) of these sera (GMT = 10.9, range = < 4-32). Moreover, IgG and IgM responses could not be distinguished using CF. Thus the sensitivity of these three techniques is greatest for ELISA. Additionally, a study of sequential sera from 3 patients with acute Ad infection disclosed seroconversion using all three methods. Both the ELISA and IF techniques permit the detection of transition from IgM to IgG, whereas CF only detects conversion from seronegativity to seropositivity. Finally, preliminary data suggest that the IgM response as measured by ELISA is specific for subgroups or types of Ad. This newly devised ELISA may be useful for detecting Ad infections.

Original languageEnglish (US)
Pages (from-to)321-332
Number of pages12
JournalJournal of Virological Methods
Volume23
Issue number3
DOIs
StatePublished - Jan 1 1989

Fingerprint

Adenoviridae Infections
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Adenoviridae
Antibodies
Immunoglobulin G
Serum
Immunosorbent Techniques
Complement Fixation Tests
Indirect Fluorescent Antibody Technique
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Virology

Cite this

Enzyme-linked immunosorbent assay (ELISA) for detecting antibodies in sera of patients with adenovirus infection. / Thiele, Geoffrey Milton; Okano, Motohiko; Purtilo, David T.

In: Journal of Virological Methods, Vol. 23, No. 3, 01.01.1989, p. 321-332.

Research output: Contribution to journalArticle

@article{41f7f01ce82242099242149582d2ae00,
title = "Enzyme-linked immunosorbent assay (ELISA) for detecting antibodies in sera of patients with adenovirus infection",
abstract = "The 41 distinct antigenic types of adenoviruses (Ads) are responsible for a broad spectrum of diseases in humans. We have developed an enzyme-linked immunosorbent assay (ELISA) using adenovirus (Ad) infected MRC-5 cells for detecting IgG and IgM antibodies to Ads. Using the ELISA, we detected IgG antibodies in 100{\%} ( 20 20) of sera from normal adults (geometric mean titer, GMT = 1840.8, range = 40-20,480) and IgM antibodies in 3 of 20 sera (15{\%}) with a GMT of 25.1. Our indirect immunofluorescence (IF) technique also detected IgG antibodies in 100{\%}) of these sera (GMT = 248.3, range = 40-5,120) and IgM antibodies in the 3 samples reactive in ELISA (GMT = 20.0, range = < 5-40). In contrast, the complement fixation (CF) test detected antibodies to Ads in only 65{\%} ( 13 20) of these sera (GMT = 10.9, range = < 4-32). Moreover, IgG and IgM responses could not be distinguished using CF. Thus the sensitivity of these three techniques is greatest for ELISA. Additionally, a study of sequential sera from 3 patients with acute Ad infection disclosed seroconversion using all three methods. Both the ELISA and IF techniques permit the detection of transition from IgM to IgG, whereas CF only detects conversion from seronegativity to seropositivity. Finally, preliminary data suggest that the IgM response as measured by ELISA is specific for subgroups or types of Ad. This newly devised ELISA may be useful for detecting Ad infections.",
author = "Thiele, {Geoffrey Milton} and Motohiko Okano and Purtilo, {David T.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0166-0934(89)90164-X",
language = "English (US)",
volume = "23",
pages = "321--332",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Enzyme-linked immunosorbent assay (ELISA) for detecting antibodies in sera of patients with adenovirus infection

AU - Thiele, Geoffrey Milton

AU - Okano, Motohiko

AU - Purtilo, David T.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The 41 distinct antigenic types of adenoviruses (Ads) are responsible for a broad spectrum of diseases in humans. We have developed an enzyme-linked immunosorbent assay (ELISA) using adenovirus (Ad) infected MRC-5 cells for detecting IgG and IgM antibodies to Ads. Using the ELISA, we detected IgG antibodies in 100% ( 20 20) of sera from normal adults (geometric mean titer, GMT = 1840.8, range = 40-20,480) and IgM antibodies in 3 of 20 sera (15%) with a GMT of 25.1. Our indirect immunofluorescence (IF) technique also detected IgG antibodies in 100%) of these sera (GMT = 248.3, range = 40-5,120) and IgM antibodies in the 3 samples reactive in ELISA (GMT = 20.0, range = < 5-40). In contrast, the complement fixation (CF) test detected antibodies to Ads in only 65% ( 13 20) of these sera (GMT = 10.9, range = < 4-32). Moreover, IgG and IgM responses could not be distinguished using CF. Thus the sensitivity of these three techniques is greatest for ELISA. Additionally, a study of sequential sera from 3 patients with acute Ad infection disclosed seroconversion using all three methods. Both the ELISA and IF techniques permit the detection of transition from IgM to IgG, whereas CF only detects conversion from seronegativity to seropositivity. Finally, preliminary data suggest that the IgM response as measured by ELISA is specific for subgroups or types of Ad. This newly devised ELISA may be useful for detecting Ad infections.

AB - The 41 distinct antigenic types of adenoviruses (Ads) are responsible for a broad spectrum of diseases in humans. We have developed an enzyme-linked immunosorbent assay (ELISA) using adenovirus (Ad) infected MRC-5 cells for detecting IgG and IgM antibodies to Ads. Using the ELISA, we detected IgG antibodies in 100% ( 20 20) of sera from normal adults (geometric mean titer, GMT = 1840.8, range = 40-20,480) and IgM antibodies in 3 of 20 sera (15%) with a GMT of 25.1. Our indirect immunofluorescence (IF) technique also detected IgG antibodies in 100%) of these sera (GMT = 248.3, range = 40-5,120) and IgM antibodies in the 3 samples reactive in ELISA (GMT = 20.0, range = < 5-40). In contrast, the complement fixation (CF) test detected antibodies to Ads in only 65% ( 13 20) of these sera (GMT = 10.9, range = < 4-32). Moreover, IgG and IgM responses could not be distinguished using CF. Thus the sensitivity of these three techniques is greatest for ELISA. Additionally, a study of sequential sera from 3 patients with acute Ad infection disclosed seroconversion using all three methods. Both the ELISA and IF techniques permit the detection of transition from IgM to IgG, whereas CF only detects conversion from seronegativity to seropositivity. Finally, preliminary data suggest that the IgM response as measured by ELISA is specific for subgroups or types of Ad. This newly devised ELISA may be useful for detecting Ad infections.

UR - http://www.scopus.com/inward/record.url?scp=0024507066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024507066&partnerID=8YFLogxK

U2 - 10.1016/0166-0934(89)90164-X

DO - 10.1016/0166-0934(89)90164-X

M3 - Article

VL - 23

SP - 321

EP - 332

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 3

ER -