Enzymatic Hydrolysis Does Not Reduce the Biological Reactivity of Soybean Proteins for All Allergic Subjects

Rakhi Panda, Afua O. Tetteh, Siddanakoppalu N. Pramod, Richard E. Goodman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity of soybean products. In this study the allergenicity of soybean protein isolates (SPI) hydrolyzed by Alcalase, trypsin, chymotrypsin, bromelain, or papain was evaluated by IgE immunoblots using eight soybean-allergic patient sera. The biological relevance of IgE binding was evaluated by a functional assay using a humanized rat basophilic leukemia (hRBL) cell line and serum from one subject. Results indicated that hydrolysis of SPI by the enzymes did not reduce the allergenicity, and hydrolysis by chymotrypsin or bromelain has the potential to increase the allergenicity of SPI. Two-dimensional (2D) immunoblot and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the chymotrypsin-hydrolyzed samples indicated fragments of β-conglycinin protein are responsible for the apparent higher allergenic potential of digested SPI.

Original languageEnglish (US)
Pages (from-to)9629-9639
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number43
DOIs
StatePublished - Oct 8 2015

Fingerprint

allergenicity
Soybean Proteins
Enzymatic hydrolysis
enzymatic hydrolysis
soy protein
protein isolates
Hydrolysis
chymotrypsin
bromelains
Bromelains
Chymotrypsin
Soybeans
Immunoglobulin E
hydrolysis
Subtilisins
Functional Food
soybean products
Papain
papain
subtilisin

Keywords

  • IgE binding
  • enzymatic hydrolysis
  • functional assay
  • soybean

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Enzymatic Hydrolysis Does Not Reduce the Biological Reactivity of Soybean Proteins for All Allergic Subjects. / Panda, Rakhi; Tetteh, Afua O.; Pramod, Siddanakoppalu N.; Goodman, Richard E.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 43, 08.10.2015, p. 9629-9639.

Research output: Contribution to journalArticle

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