Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts

Timothy P. Carr, Catharine J. Andresen, Lawrence L. Rudel

Research output: Contribution to journalArticle

443 Citations (Scopus)

Abstract

Triglyceride, free cholesterol, and total cholesterol were quantified in lipid extracts of liver and thoracic aorta from nonhuman primates using commercially available enzymatic reagents. Lipids were solubilized in water by the addition of Triton X-100. Results of the enzymatic assays compared favorably with chemical assays of lipids separated by thin-layer chromatography. In addition to saving time, the present method has the advantage of measuring each lipid class from a single sample preparation. Furthermore, the procedure has been adapted for use with microtiter plates that conserve both sample and reagent.

Original languageEnglish (US)
Pages (from-to)39-42
Number of pages4
JournalClinical Biochemistry
Volume26
Issue number1
DOIs
StatePublished - Feb 1993

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Tissue Extracts
Triglycerides
Cholesterol
Tissue
Lipids
Assays
Liver Extracts
Thin layer chromatography
Octoxynol
Enzyme Assays
Thin Layer Chromatography
Thoracic Aorta
Primates
Water

Keywords

  • cholesterol
  • cholesteryl esters
  • enzymatic assay
  • tissue lipids
  • triglycerides

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts. / Carr, Timothy P.; Andresen, Catharine J.; Rudel, Lawrence L.

In: Clinical Biochemistry, Vol. 26, No. 1, 02.1993, p. 39-42.

Research output: Contribution to journalArticle

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