Enumeration of T cells, B cells and monocytes in the peripheral blood of normal and lymphocytotic cattle

P. S. Paul, D. R. Senogles, C. C. Muscoplat, D. W. Johnson

Research output: Contribution to journalArticle

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Abstract

The rosette-forming capacity of bovine peripheral blood lymphocytes (PBL) was determined with dextran and 2-aminoethylisothiouronium bromide (AET)-treated sheep erythocytes (SRBC). Both dextran and AET-enhanced rosette formation; however, AET-treated SRBC detected a larger percentage of rosette-forming cells and thus was used in this study. The specificity of rosette formation by bovine thymus-derived (T) lymphocytes was shown by (1) demonstration of rosettes and surface-membrane immunoglobulins (sIg) on different cells in PBL and nylon-wool fractionated lymphocyte populations and (2) rosette formation by a large percentage (83-90%) of thymocytes from three bovine foetuses and two 14-month-old heifers. A procedure was also developed to identify bovine monocytes by latex phagocytosis and 10-30% latex-ingesting cells were detected in PBL preparations isolated by Ficoll-Hypaque flotation. The frequency of sIg-bearing latex-ingesting, and sIg-bearing latex non-ingesting cells in bovine peripheral blood was also determined. These procedures were utilized to determine the distribution of T and bone-marrow derived (B) lymphocytes in peripheral blood of normal and lymphocytotic cattle. PBL from twenty normal cattle contained approximately 63% T and 11% B (sIg+latex non-ingesting) lymphocytes. In peripheral blood of three cattle with persistent lymphocytosis, a prodromal stage of bovine leukaemia, the percentage of B cells was elevated approximately to 59% whereas T lymphocytes decreased to 35%, thus providing additional evidence that persistent lymphocytosis is a B-cell disease

Original languageEnglish (US)
Pages (from-to)306-316
Number of pages11
JournalClinical and Experimental Immunology
Volume35
Issue number2
StatePublished - Jan 1 1979

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Monocytes
B-Lymphocytes
Latex
T-Lymphocytes
B-Cell Antigen Receptors
Lymphocytes
Rosette Formation
Bromides
Lymphocytosis
Membranes
Dextrans
Enzootic Bovine Leukosis
Prodromal Symptoms
Diatrizoate
Ficoll
Wool
Nylons
Thymocytes
Phagocytosis
Thymus Gland

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Enumeration of T cells, B cells and monocytes in the peripheral blood of normal and lymphocytotic cattle. / Paul, P. S.; Senogles, D. R.; Muscoplat, C. C.; Johnson, D. W.

In: Clinical and Experimental Immunology, Vol. 35, No. 2, 01.01.1979, p. 306-316.

Research output: Contribution to journalArticle

Paul, P. S. ; Senogles, D. R. ; Muscoplat, C. C. ; Johnson, D. W. / Enumeration of T cells, B cells and monocytes in the peripheral blood of normal and lymphocytotic cattle. In: Clinical and Experimental Immunology. 1979 ; Vol. 35, No. 2. pp. 306-316.
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