Entrapment of proteins in glycogen-capped and hydrazide-activated supports

Abby J. Jackson, Hai Xuan, David S Hage

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (±1). mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150. kDa.

Original languageEnglish (US)
Pages (from-to)106-108
Number of pages3
JournalAnalytical Biochemistry
Volume404
Issue number1
DOIs
StatePublished - Sep 1 2010

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Glycogen
Serum Albumin
Proteins
Equilibrium constants
Warfarin
Protein Binding
Silicon Dioxide
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

Entrapment of proteins in glycogen-capped and hydrazide-activated supports. / Jackson, Abby J.; Xuan, Hai; Hage, David S.

In: Analytical Biochemistry, Vol. 404, No. 1, 01.09.2010, p. 106-108.

Research output: Contribution to journalArticle

Jackson, Abby J. ; Xuan, Hai ; Hage, David S. / Entrapment of proteins in glycogen-capped and hydrazide-activated supports. In: Analytical Biochemistry. 2010 ; Vol. 404, No. 1. pp. 106-108.
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