Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells

M. L. McKee, A. R. Melton-Celsa, R. A. Moxley, D. H. Francis, A. D. O'Brien

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

In a previous study, enterohemorrhagic Escherichia coli (EHEC) O157:H7 with a deletion and insertion in the eaeA gene encoding intimin was used to establish that intimin is required for the organism to attach to and efface microvilli in the piglet intestine (M. S. Donnenberg, S. Tzipori, M. L. McKee, A. D. O'Brien, J. Alroy, and J. B. Kaper, J. Clin. Invest. 92:1418- 1424, 1993). However, in the same investigation, a role for intimin in EHEC adherence to HEp-2 cells could not be definitively demonstrated. To analyze the basis for this discrepancy, we constructed an in-frame deletion of eaeA and compared the adherence capacity of this mutant with that of the wild- type strain in vitro and in vivo. We observed a direct correlation between the requisite for intimin in EHEC O157:H7 colonization of the gnotobiotic piglet intestine and adherence of the bacterium to HEp-2 cells. The in vitro- in vivo correlation lends credence to the use of the HEp-2 cell adherence model for further study of the intimin protein.

Original languageEnglish (US)
Pages (from-to)3739-3744
Number of pages6
JournalInfection and immunity
Volume63
Issue number9
StatePublished - Jan 1 1995

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Germ-Free Life
Enterohemorrhagic Escherichia coli
Escherichia coli O157
Intestines
Swine
Microvilli
Bacteria
Genes
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. / McKee, M. L.; Melton-Celsa, A. R.; Moxley, R. A.; Francis, D. H.; O'Brien, A. D.

In: Infection and immunity, Vol. 63, No. 9, 01.01.1995, p. 3739-3744.

Research output: Contribution to journalArticle

McKee, M. L. ; Melton-Celsa, A. R. ; Moxley, R. A. ; Francis, D. H. ; O'Brien, A. D. / Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. In: Infection and immunity. 1995 ; Vol. 63, No. 9. pp. 3739-3744.
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