Enrichment of malondialdehyde-acetaldehyde antibody in the rheumatoid arthritis joint

Ted R Mikuls, Michael J. Duryee, Rafid Rahman, Daniel R Anderson, Harlan R. Sayles, Andrew Hollins, Kaleb D Michaud, Frederick Wolfe, Geoffrey E. Thiele, Jeremy Sokolove, William H. Robinson, Nithya Lingampalli, Anthony P. Nicholas, Geoffrey A Talmon, Kaihong Su, Matthew C Zimmerman, Lynell Warren Klassen, Geoffrey Milton Thiele

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To characterize the expression of malondialdehdye-acetaldehyde (MAA) adducts and anti-MAA antibody in articular tissues and serum of patients with RA.

Methods: Paired sera and SF were examined from 29 RA and 13 OA patients. Anti-MAA antibody, RF, ACPA and total immunoglobulin were quantified. SF-serum measures were compared within and between disease groups. The presence and co-localization of MAA, citrulline and select leukocyte antigens in RA and OA synovial tissues were examined using immunohistochemistry.

Results: Circulating and SF anti-MAA antibody concentrations were higher in RA vs OA by 1.5- to 5-fold. IgG (P < 0.001), IgM (P = 0.006) and IgA (P = 0.036) anti-MAA antibodies were higher in paired RA SF than serum, differences not observed for total immunoglobulin, RF or ACPA. In RA synovial tissues, co-localization of MAA with citrulline and CD19+ or CD27+ B cells was demonstrated and was much higher in magnitude than MAA or citrulline co-localization with T cells, monocytes, macrophages or dendritic cells (P < 0.01).

Conclusion: Anti-MAA antibodies are present in higher concentrations in the RA joint compared with sera, a finding not observed for other disease-related autoantibodies. Co-localization of MAA and citrulline with mature B cells, coupled with the local enrichment of anti-MAA immune responses, implicates MAA-adduct formation in local autoantibody production.

Original languageEnglish (US)
Pages (from-to)1794-1803
Number of pages10
JournalRheumatology (Oxford, England)
Volume56
Issue number10
DOIs
StatePublished - Oct 1 2017

Fingerprint

Acetaldehyde
Malondialdehyde
Rheumatoid Arthritis
Joints
Antibodies
Citrulline
Serum
Autoantibodies
Immunoglobulins
B-Lymphocytes
HLA Antigens
Dendritic Cells
Immunoglobulin A
Immunoglobulin M
Monocytes
Immunoglobulin G
Immunohistochemistry
Macrophages

Keywords

  • anti-citrullinated protein antibody (ACPA)
  • autoimmunity
  • malondialdehyde–acetaldehyde adducts
  • oxidative stress
  • rheumatoid arthritis
  • synovial fluid

ASJC Scopus subject areas

  • Rheumatology
  • Pharmacology (medical)

Cite this

Enrichment of malondialdehyde-acetaldehyde antibody in the rheumatoid arthritis joint. / Mikuls, Ted R; Duryee, Michael J.; Rahman, Rafid; Anderson, Daniel R; Sayles, Harlan R.; Hollins, Andrew; Michaud, Kaleb D; Wolfe, Frederick; Thiele, Geoffrey E.; Sokolove, Jeremy; Robinson, William H.; Lingampalli, Nithya; Nicholas, Anthony P.; Talmon, Geoffrey A; Su, Kaihong; Zimmerman, Matthew C; Klassen, Lynell Warren; Thiele, Geoffrey Milton.

In: Rheumatology (Oxford, England), Vol. 56, No. 10, 01.10.2017, p. 1794-1803.

Research output: Contribution to journalArticle

Mikuls, TR, Duryee, MJ, Rahman, R, Anderson, DR, Sayles, HR, Hollins, A, Michaud, KD, Wolfe, F, Thiele, GE, Sokolove, J, Robinson, WH, Lingampalli, N, Nicholas, AP, Talmon, GA, Su, K, Zimmerman, MC, Klassen, LW & Thiele, GM 2017, 'Enrichment of malondialdehyde-acetaldehyde antibody in the rheumatoid arthritis joint', Rheumatology (Oxford, England), vol. 56, no. 10, pp. 1794-1803. https://doi.org/10.1093/rheumatology/kex212
Mikuls, Ted R ; Duryee, Michael J. ; Rahman, Rafid ; Anderson, Daniel R ; Sayles, Harlan R. ; Hollins, Andrew ; Michaud, Kaleb D ; Wolfe, Frederick ; Thiele, Geoffrey E. ; Sokolove, Jeremy ; Robinson, William H. ; Lingampalli, Nithya ; Nicholas, Anthony P. ; Talmon, Geoffrey A ; Su, Kaihong ; Zimmerman, Matthew C ; Klassen, Lynell Warren ; Thiele, Geoffrey Milton. / Enrichment of malondialdehyde-acetaldehyde antibody in the rheumatoid arthritis joint. In: Rheumatology (Oxford, England). 2017 ; Vol. 56, No. 10. pp. 1794-1803.
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abstract = "Objective: To characterize the expression of malondialdehdye-acetaldehyde (MAA) adducts and anti-MAA antibody in articular tissues and serum of patients with RA.Methods: Paired sera and SF were examined from 29 RA and 13 OA patients. Anti-MAA antibody, RF, ACPA and total immunoglobulin were quantified. SF-serum measures were compared within and between disease groups. The presence and co-localization of MAA, citrulline and select leukocyte antigens in RA and OA synovial tissues were examined using immunohistochemistry.Results: Circulating and SF anti-MAA antibody concentrations were higher in RA vs OA by 1.5- to 5-fold. IgG (P < 0.001), IgM (P = 0.006) and IgA (P = 0.036) anti-MAA antibodies were higher in paired RA SF than serum, differences not observed for total immunoglobulin, RF or ACPA. In RA synovial tissues, co-localization of MAA with citrulline and CD19+ or CD27+ B cells was demonstrated and was much higher in magnitude than MAA or citrulline co-localization with T cells, monocytes, macrophages or dendritic cells (P < 0.01).Conclusion: Anti-MAA antibodies are present in higher concentrations in the RA joint compared with sera, a finding not observed for other disease-related autoantibodies. Co-localization of MAA and citrulline with mature B cells, coupled with the local enrichment of anti-MAA immune responses, implicates MAA-adduct formation in local autoantibody production.",
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T1 - Enrichment of malondialdehyde-acetaldehyde antibody in the rheumatoid arthritis joint

AU - Mikuls, Ted R

AU - Duryee, Michael J.

AU - Rahman, Rafid

AU - Anderson, Daniel R

AU - Sayles, Harlan R.

AU - Hollins, Andrew

AU - Michaud, Kaleb D

AU - Wolfe, Frederick

AU - Thiele, Geoffrey E.

AU - Sokolove, Jeremy

AU - Robinson, William H.

AU - Lingampalli, Nithya

AU - Nicholas, Anthony P.

AU - Talmon, Geoffrey A

AU - Su, Kaihong

AU - Zimmerman, Matthew C

AU - Klassen, Lynell Warren

AU - Thiele, Geoffrey Milton

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Objective: To characterize the expression of malondialdehdye-acetaldehyde (MAA) adducts and anti-MAA antibody in articular tissues and serum of patients with RA.Methods: Paired sera and SF were examined from 29 RA and 13 OA patients. Anti-MAA antibody, RF, ACPA and total immunoglobulin were quantified. SF-serum measures were compared within and between disease groups. The presence and co-localization of MAA, citrulline and select leukocyte antigens in RA and OA synovial tissues were examined using immunohistochemistry.Results: Circulating and SF anti-MAA antibody concentrations were higher in RA vs OA by 1.5- to 5-fold. IgG (P < 0.001), IgM (P = 0.006) and IgA (P = 0.036) anti-MAA antibodies were higher in paired RA SF than serum, differences not observed for total immunoglobulin, RF or ACPA. In RA synovial tissues, co-localization of MAA with citrulline and CD19+ or CD27+ B cells was demonstrated and was much higher in magnitude than MAA or citrulline co-localization with T cells, monocytes, macrophages or dendritic cells (P < 0.01).Conclusion: Anti-MAA antibodies are present in higher concentrations in the RA joint compared with sera, a finding not observed for other disease-related autoantibodies. Co-localization of MAA and citrulline with mature B cells, coupled with the local enrichment of anti-MAA immune responses, implicates MAA-adduct formation in local autoantibody production.

AB - Objective: To characterize the expression of malondialdehdye-acetaldehyde (MAA) adducts and anti-MAA antibody in articular tissues and serum of patients with RA.Methods: Paired sera and SF were examined from 29 RA and 13 OA patients. Anti-MAA antibody, RF, ACPA and total immunoglobulin were quantified. SF-serum measures were compared within and between disease groups. The presence and co-localization of MAA, citrulline and select leukocyte antigens in RA and OA synovial tissues were examined using immunohistochemistry.Results: Circulating and SF anti-MAA antibody concentrations were higher in RA vs OA by 1.5- to 5-fold. IgG (P < 0.001), IgM (P = 0.006) and IgA (P = 0.036) anti-MAA antibodies were higher in paired RA SF than serum, differences not observed for total immunoglobulin, RF or ACPA. In RA synovial tissues, co-localization of MAA with citrulline and CD19+ or CD27+ B cells was demonstrated and was much higher in magnitude than MAA or citrulline co-localization with T cells, monocytes, macrophages or dendritic cells (P < 0.01).Conclusion: Anti-MAA antibodies are present in higher concentrations in the RA joint compared with sera, a finding not observed for other disease-related autoantibodies. Co-localization of MAA and citrulline with mature B cells, coupled with the local enrichment of anti-MAA immune responses, implicates MAA-adduct formation in local autoantibody production.

KW - anti-citrullinated protein antibody (ACPA)

KW - autoimmunity

KW - malondialdehyde–acetaldehyde adducts

KW - oxidative stress

KW - rheumatoid arthritis

KW - synovial fluid

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