Enhancement of polyhydroxybutyrate (PHB) production by 10-fold from alkaline pretreatment liquor with an oxidative enzyme-mediator-surfactant system under Plackett-Burman and central composite designs

Mengxing Li, Kent Eskridge, Enshi Liu, Mark Wilkins

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, Plackett-Burman and central composite designs were applied to improve polyhydroxybutyrate (PHB) production from alkaline pretreatment liquor (APL) by Cupriavidus necator DSM 545 using a supplement system consisting of oxidative enzymes (laccase, aryl alcohol oxidase (AAO)), mediators (ABTS, HOBT), DMSO, silica nanoparticle Aerosol R816 and surfactant Tween 80. First, screening experiments under Plackett-Burman design showed R816, ABTS and Tween 80 could significantly enhance PHB production. Additional experiments showed that HOBT and DMSO could be removed, and laccase and AAO were needed to remain in the system. Second, a central composite design was applied to obtain the optimum supplemental levels of R816, ABTS and Tween 80. Under optimum conditions, theoretical maximum PHB production (1.9 g/L) was close to experimental PHB production (2.1 g/L). With the supplement system, a 10-fold increase was achieved compared to PHB production (0.2 g/L) without any supplements.

Original languageEnglish (US)
Pages (from-to)99-106
Number of pages8
JournalBioresource Technology
Volume281
DOIs
StatePublished - Jun 2019

Fingerprint

Surface-Active Agents
surfactant
Surface active agents
Enzymes
Polysorbates
enzyme
fold
alcohol
Composite materials
Laccase
Dimethyl Sulfoxide
Alcohols
silica
experiment
aerosol
Aerosols
Silicon Dioxide
Screening
Experiments
Silica

Keywords

  • 10-Fold increase
  • Alkaline pretreatment liquor (APL)
  • Central composite design
  • Plackett-Burman design
  • Polyhydroxybutyrate

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

@article{add35f0e54c64ec284b5ac8e86633917,
title = "Enhancement of polyhydroxybutyrate (PHB) production by 10-fold from alkaline pretreatment liquor with an oxidative enzyme-mediator-surfactant system under Plackett-Burman and central composite designs",
abstract = "In this study, Plackett-Burman and central composite designs were applied to improve polyhydroxybutyrate (PHB) production from alkaline pretreatment liquor (APL) by Cupriavidus necator DSM 545 using a supplement system consisting of oxidative enzymes (laccase, aryl alcohol oxidase (AAO)), mediators (ABTS, HOBT), DMSO, silica nanoparticle Aerosol R816 and surfactant Tween 80. First, screening experiments under Plackett-Burman design showed R816, ABTS and Tween 80 could significantly enhance PHB production. Additional experiments showed that HOBT and DMSO could be removed, and laccase and AAO were needed to remain in the system. Second, a central composite design was applied to obtain the optimum supplemental levels of R816, ABTS and Tween 80. Under optimum conditions, theoretical maximum PHB production (1.9 g/L) was close to experimental PHB production (2.1 g/L). With the supplement system, a 10-fold increase was achieved compared to PHB production (0.2 g/L) without any supplements.",
keywords = "10-Fold increase, Alkaline pretreatment liquor (APL), Central composite design, Plackett-Burman design, Polyhydroxybutyrate",
author = "Mengxing Li and Kent Eskridge and Enshi Liu and Mark Wilkins",
year = "2019",
month = "6",
doi = "10.1016/j.biortech.2019.02.045",
language = "English (US)",
volume = "281",
pages = "99--106",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Enhancement of polyhydroxybutyrate (PHB) production by 10-fold from alkaline pretreatment liquor with an oxidative enzyme-mediator-surfactant system under Plackett-Burman and central composite designs

AU - Li, Mengxing

AU - Eskridge, Kent

AU - Liu, Enshi

AU - Wilkins, Mark

PY - 2019/6

Y1 - 2019/6

N2 - In this study, Plackett-Burman and central composite designs were applied to improve polyhydroxybutyrate (PHB) production from alkaline pretreatment liquor (APL) by Cupriavidus necator DSM 545 using a supplement system consisting of oxidative enzymes (laccase, aryl alcohol oxidase (AAO)), mediators (ABTS, HOBT), DMSO, silica nanoparticle Aerosol R816 and surfactant Tween 80. First, screening experiments under Plackett-Burman design showed R816, ABTS and Tween 80 could significantly enhance PHB production. Additional experiments showed that HOBT and DMSO could be removed, and laccase and AAO were needed to remain in the system. Second, a central composite design was applied to obtain the optimum supplemental levels of R816, ABTS and Tween 80. Under optimum conditions, theoretical maximum PHB production (1.9 g/L) was close to experimental PHB production (2.1 g/L). With the supplement system, a 10-fold increase was achieved compared to PHB production (0.2 g/L) without any supplements.

AB - In this study, Plackett-Burman and central composite designs were applied to improve polyhydroxybutyrate (PHB) production from alkaline pretreatment liquor (APL) by Cupriavidus necator DSM 545 using a supplement system consisting of oxidative enzymes (laccase, aryl alcohol oxidase (AAO)), mediators (ABTS, HOBT), DMSO, silica nanoparticle Aerosol R816 and surfactant Tween 80. First, screening experiments under Plackett-Burman design showed R816, ABTS and Tween 80 could significantly enhance PHB production. Additional experiments showed that HOBT and DMSO could be removed, and laccase and AAO were needed to remain in the system. Second, a central composite design was applied to obtain the optimum supplemental levels of R816, ABTS and Tween 80. Under optimum conditions, theoretical maximum PHB production (1.9 g/L) was close to experimental PHB production (2.1 g/L). With the supplement system, a 10-fold increase was achieved compared to PHB production (0.2 g/L) without any supplements.

KW - 10-Fold increase

KW - Alkaline pretreatment liquor (APL)

KW - Central composite design

KW - Plackett-Burman design

KW - Polyhydroxybutyrate

UR - http://www.scopus.com/inward/record.url?scp=85061903098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061903098&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2019.02.045

DO - 10.1016/j.biortech.2019.02.045

M3 - Article

C2 - 30807996

AN - SCOPUS:85061903098

VL - 281

SP - 99

EP - 106

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -