Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts

M. G. Brattain, D. E. Brattain, A. M. Sarrif, L. J. McRae, W. D. Fine, J. G. Hawkins

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency be cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.

Original languageEnglish (US)
Pages (from-to)767-771
Number of pages5
JournalJournal of the National Cancer Institute
Volume69
Issue number4
StatePublished - Dec 17 1982

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Feeder Cells
Tumor Cell Line
Colon
Fibroblasts
Growth
Heterografts
Cultured Cells
Cell Line

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Brattain, M. G., Brattain, D. E., Sarrif, A. M., McRae, L. J., Fine, W. D., & Hawkins, J. G. (1982). Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. Journal of the National Cancer Institute, 69(4), 767-771.

Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. / Brattain, M. G.; Brattain, D. E.; Sarrif, A. M.; McRae, L. J.; Fine, W. D.; Hawkins, J. G.

In: Journal of the National Cancer Institute, Vol. 69, No. 4, 17.12.1982, p. 767-771.

Research output: Contribution to journalArticle

Brattain, MG, Brattain, DE, Sarrif, AM, McRae, LJ, Fine, WD & Hawkins, JG 1982, 'Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts', Journal of the National Cancer Institute, vol. 69, no. 4, pp. 767-771.
Brattain MG, Brattain DE, Sarrif AM, McRae LJ, Fine WD, Hawkins JG. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. Journal of the National Cancer Institute. 1982 Dec 17;69(4):767-771.
Brattain, M. G. ; Brattain, D. E. ; Sarrif, A. M. ; McRae, L. J. ; Fine, W. D. ; Hawkins, J. G. / Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. In: Journal of the National Cancer Institute. 1982 ; Vol. 69, No. 4. pp. 767-771.
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