Enhancement of autophagy during lytic replication by the Kaposi's sarcoma-associated herpesvirus replication and transcription activator

Hui Ju Wen, Zhilong Yang, You Zhou, Charles Wood

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Autophagy is one of two major degradation systems in eukaryotic cells. The degradation mechanism of autophagy is required to maintain the balance between the biosynthetic and catabolic processes and also contributes to defense against invading pathogens. Recent studies suggest that a number of viruses can evade or subvert the host cell autophagic pathway to enhance their own replication. Here, we investigated the effect of autophagy on the KSHV (Kaposi's sarcoma-associated herpesvirus) life cycle. We found that the inhibition of autophagy reduces KSHV lytic reactivation from latency, and an enhancement of autophagy can be detected during KSHV lytic replication. In addition, RTA (replication and transcription activator), an essential viral protein for KSHV lytic reactivation, is able to enhance the autophagic process, leading to an increase in the number of autophagic vacuoles, an increase in the level of the lipidated LC3 protein, and the formation of autolysosomes. Moreover, the inhibition of autophagy affects RTA-mediated lytic gene expression and viral DNA replication. These results suggest that RTA increases autophagy activation to facilitate KSHV lytic replication. This is the first report demonstrating that autophagy is involved in the lytic reactivation of KSHV.

Original languageEnglish (US)
Pages (from-to)7448-7458
Number of pages11
JournalJournal of virology
Volume84
Issue number15
DOIs
StatePublished - Aug 1 2010

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Human herpesvirus 8
Human Herpesvirus 8
autophagy
Autophagy
transcription (genetics)
degradation
viral proteins
Viral DNA
Eukaryotic Cells
Viral Proteins
DNA replication
Vacuoles
Life Cycle Stages
DNA Replication
vacuoles
eukaryotic cells
Viruses
Gene Expression
gene expression
viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Enhancement of autophagy during lytic replication by the Kaposi's sarcoma-associated herpesvirus replication and transcription activator. / Wen, Hui Ju; Yang, Zhilong; Zhou, You; Wood, Charles.

In: Journal of virology, Vol. 84, No. 15, 01.08.2010, p. 7448-7458.

Research output: Contribution to journalArticle

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