Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes

G. Luigi Spadoni, Anna Spagnoli, S. Cianfarani, D. Del Principe, A. Menichelli, S. Di Giulio, B. Boscherini

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The oxidative metabolic burst of mononuclear and polymorphonuclear phagocytes can be stimulated to produce free oxygen radicals. Several substances can enhance this respiratory burst activity by a priming action: recently growth hormone (rat and porcine) was demonstrated to act as a priming agent on rat peritoneal and on porcine alveolar macrophages. In our study we wanted to verify whether also human GH had a similar priming action on homologous cells, in particular on polymorphonuclear leukocytes. To determine the oxidative activity of polymorphonuclear leukocytes, after stimulation with phorbol myristate acetate, a flow-cytometric assay was employed whith registered the intracellular formation of highly fluorescent products as indicators for the intracellular formation of hydrogen peroxide. The incubation of phorbol myristate acetate-stimulated polymorphonuclear leukocytes with GH resulted in a time-dependent and dose-dependent increase in fluorescence, thus demonstrating that human GH enhances in vitro the oxidative metabolic burst of these cells. The action of GH appeared to be significant after 30 min of incubation, was maximal at 60 min, and decreased after 90 min. After one hour of incubation, the first significant variation of fluorescence appeared with GH at a concentration of 50 μg/l. The maximum effect was seen at 100 μg/l with no further increase. Specificity of GH action was demonstrated by the inhibition of its effect by the addition of monoclonal antibodies to GH.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalActa Endocrinologica
Volume124
Issue number5
StatePublished - Jan 1 1991

Fingerprint

Respiratory Burst
Phorbol Esters
Growth Hormone
Neutrophils
Tetradecanoylphorbol Acetate
Swine
Fluorescence
Alveolar Macrophages
Phagocytes
Hydrogen Peroxide
Free Radicals
Reactive Oxygen Species
Monoclonal Antibodies

ASJC Scopus subject areas

  • Endocrinology

Cite this

Luigi Spadoni, G., Spagnoli, A., Cianfarani, S., Del Principe, D., Menichelli, A., Di Giulio, S., & Boscherini, B. (1991). Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes. Acta Endocrinologica, 124(5), 589-594.

Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes. / Luigi Spadoni, G.; Spagnoli, Anna; Cianfarani, S.; Del Principe, D.; Menichelli, A.; Di Giulio, S.; Boscherini, B.

In: Acta Endocrinologica, Vol. 124, No. 5, 01.01.1991, p. 589-594.

Research output: Contribution to journalArticle

Luigi Spadoni, G, Spagnoli, A, Cianfarani, S, Del Principe, D, Menichelli, A, Di Giulio, S & Boscherini, B 1991, 'Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes', Acta Endocrinologica, vol. 124, no. 5, pp. 589-594.
Luigi Spadoni G, Spagnoli A, Cianfarani S, Del Principe D, Menichelli A, Di Giulio S et al. Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes. Acta Endocrinologica. 1991 Jan 1;124(5):589-594.
Luigi Spadoni, G. ; Spagnoli, Anna ; Cianfarani, S. ; Del Principe, D. ; Menichelli, A. ; Di Giulio, S. ; Boscherini, B. / Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes. In: Acta Endocrinologica. 1991 ; Vol. 124, No. 5. pp. 589-594.
@article{f27780a7f4c0482bb45b430c42713088,
title = "Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes",
abstract = "The oxidative metabolic burst of mononuclear and polymorphonuclear phagocytes can be stimulated to produce free oxygen radicals. Several substances can enhance this respiratory burst activity by a priming action: recently growth hormone (rat and porcine) was demonstrated to act as a priming agent on rat peritoneal and on porcine alveolar macrophages. In our study we wanted to verify whether also human GH had a similar priming action on homologous cells, in particular on polymorphonuclear leukocytes. To determine the oxidative activity of polymorphonuclear leukocytes, after stimulation with phorbol myristate acetate, a flow-cytometric assay was employed whith registered the intracellular formation of highly fluorescent products as indicators for the intracellular formation of hydrogen peroxide. The incubation of phorbol myristate acetate-stimulated polymorphonuclear leukocytes with GH resulted in a time-dependent and dose-dependent increase in fluorescence, thus demonstrating that human GH enhances in vitro the oxidative metabolic burst of these cells. The action of GH appeared to be significant after 30 min of incubation, was maximal at 60 min, and decreased after 90 min. After one hour of incubation, the first significant variation of fluorescence appeared with GH at a concentration of 50 μg/l. The maximum effect was seen at 100 μg/l with no further increase. Specificity of GH action was demonstrated by the inhibition of its effect by the addition of monoclonal antibodies to GH.",
author = "{Luigi Spadoni}, G. and Anna Spagnoli and S. Cianfarani and {Del Principe}, D. and A. Menichelli and {Di Giulio}, S. and B. Boscherini",
year = "1991",
month = "1",
day = "1",
language = "English (US)",
volume = "124",
pages = "589--594",
journal = "European Journal of Endocrinology",
issn = "0804-4643",
publisher = "BioScientifica Ltd.",
number = "5",

}

TY - JOUR

T1 - Enhancement by growth hormone of phorbol diester-stimulated respiratory burst in human polymorphonuclear leukocytes

AU - Luigi Spadoni, G.

AU - Spagnoli, Anna

AU - Cianfarani, S.

AU - Del Principe, D.

AU - Menichelli, A.

AU - Di Giulio, S.

AU - Boscherini, B.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The oxidative metabolic burst of mononuclear and polymorphonuclear phagocytes can be stimulated to produce free oxygen radicals. Several substances can enhance this respiratory burst activity by a priming action: recently growth hormone (rat and porcine) was demonstrated to act as a priming agent on rat peritoneal and on porcine alveolar macrophages. In our study we wanted to verify whether also human GH had a similar priming action on homologous cells, in particular on polymorphonuclear leukocytes. To determine the oxidative activity of polymorphonuclear leukocytes, after stimulation with phorbol myristate acetate, a flow-cytometric assay was employed whith registered the intracellular formation of highly fluorescent products as indicators for the intracellular formation of hydrogen peroxide. The incubation of phorbol myristate acetate-stimulated polymorphonuclear leukocytes with GH resulted in a time-dependent and dose-dependent increase in fluorescence, thus demonstrating that human GH enhances in vitro the oxidative metabolic burst of these cells. The action of GH appeared to be significant after 30 min of incubation, was maximal at 60 min, and decreased after 90 min. After one hour of incubation, the first significant variation of fluorescence appeared with GH at a concentration of 50 μg/l. The maximum effect was seen at 100 μg/l with no further increase. Specificity of GH action was demonstrated by the inhibition of its effect by the addition of monoclonal antibodies to GH.

AB - The oxidative metabolic burst of mononuclear and polymorphonuclear phagocytes can be stimulated to produce free oxygen radicals. Several substances can enhance this respiratory burst activity by a priming action: recently growth hormone (rat and porcine) was demonstrated to act as a priming agent on rat peritoneal and on porcine alveolar macrophages. In our study we wanted to verify whether also human GH had a similar priming action on homologous cells, in particular on polymorphonuclear leukocytes. To determine the oxidative activity of polymorphonuclear leukocytes, after stimulation with phorbol myristate acetate, a flow-cytometric assay was employed whith registered the intracellular formation of highly fluorescent products as indicators for the intracellular formation of hydrogen peroxide. The incubation of phorbol myristate acetate-stimulated polymorphonuclear leukocytes with GH resulted in a time-dependent and dose-dependent increase in fluorescence, thus demonstrating that human GH enhances in vitro the oxidative metabolic burst of these cells. The action of GH appeared to be significant after 30 min of incubation, was maximal at 60 min, and decreased after 90 min. After one hour of incubation, the first significant variation of fluorescence appeared with GH at a concentration of 50 μg/l. The maximum effect was seen at 100 μg/l with no further increase. Specificity of GH action was demonstrated by the inhibition of its effect by the addition of monoclonal antibodies to GH.

UR - http://www.scopus.com/inward/record.url?scp=0025875011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025875011&partnerID=8YFLogxK

M3 - Article

VL - 124

SP - 589

EP - 594

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

SN - 0804-4643

IS - 5

ER -