Enhanced Immunosuppressive Activity Associated with Metastatic Lymphoma Cells

Weimin Hao, Shantaram S Joshi, Kenneth W. Brunson, Shantaram S. Joshi

Research output: Contribution to journalArticle

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Abstract

Earlier reports from our laboratory showed that Abelson virus-induced, highly malignant and liver metastatic RAW117-H10 cells, but not the parental, less metastatic RAWU7-P cells, inhibited both T-cell and B-cell mitogen-induced proliferation of syngeneic normal murine spleen cells. Similar inhibition was also noted when RAW117-H10 cell surface molecules extracted with butanol were used instead of whole tumor cells. In this report we describe the suppressive properties of the butanol-extracted RAW117-H10 cell surface molecules on other immune functions and the isolation/purification of a molecule from RAW117-H10 cell butanol extract which shows inhibitory activity. The immunosuppressive molecules also inhibit natural killer cell-mediated cytotoxicity, lymphokine-activated killer cell-mediated cytotoxicity, and bone marrow colony-forming unit-granulocyte-macrophage colony formation, but not colony-forming unit-fibroblast colony formation. The suppressive molecules inhibit interleukin 2 production by the T-lymphocytes. One of the molecules responsible for some of the immunosuppressive activity has been isolated and purified from butanol extracts of the metastatic RAW117-H10 cells by preparative isoelectrofocusing techniques. The suppressive molecule has an isoelectric point of 43 with an approximate molecular weight of 70,000. Metastatic RAW117-H10 lymphoma cells therefore express immunosuppressive molecules, which may facilitate their growth and metas-.

Original languageEnglish (US)
Pages (from-to)1921-1928
Number of pages8
JournalCancer Research
Volume53
Issue number8
StatePublished - Apr 1993

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Immunosuppressive Agents
Lymphoma
Butanols
Granulocyte-Macrophage Progenitor Cells
T-Lymphocytes
Lymphokine-Activated Killer Cells
Isoelectric Point
Cell Extracts
Mitogens
Natural Killer Cells
Interleukin-2
B-Lymphocytes
Stem Cells
Spleen
Fibroblasts
Molecular Weight
Bone Marrow
Viruses
Liver
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Enhanced Immunosuppressive Activity Associated with Metastatic Lymphoma Cells. / Hao, Weimin; Joshi, Shantaram S; Brunson, Kenneth W.; Joshi, Shantaram S.

In: Cancer Research, Vol. 53, No. 8, 04.1993, p. 1921-1928.

Research output: Contribution to journalArticle

Hao, W, Joshi, SS, Brunson, KW & Joshi, SS 1993, 'Enhanced Immunosuppressive Activity Associated with Metastatic Lymphoma Cells', Cancer Research, vol. 53, no. 8, pp. 1921-1928.
Hao, Weimin ; Joshi, Shantaram S ; Brunson, Kenneth W. ; Joshi, Shantaram S. / Enhanced Immunosuppressive Activity Associated with Metastatic Lymphoma Cells. In: Cancer Research. 1993 ; Vol. 53, No. 8. pp. 1921-1928.
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