Enhanced degradation of p53 protein in HPV-6 and BPV-1 E6-immortalized human mammary epithelial cells

Vimla Band, Sorab Dalal, Laurie Delmolino, Elliot J. Androphy

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Normal mammary epithelial cells are efficiently immortalized by the E6 gene of human papillomavirus (HPV)-16, a virus commonly associated with cervical cancers. Surprisingly, introduction of the E6 gene from HPV-6, which is rarely found in cervical cancer, or bovine papillomavirus (BPV)-1, into normal mammary cells resulted in the generation of immortal cell lines. The establishment of HPV-6 and BPV-1 E6-immortalized cells was less efficient and required a longer period in comparison to HPV-16 E6. These HPV-6- and BPV-1 E6-immortalized cells demonstrated dramatically reduced levels of p53 protein by immunoprecipitation. While the half-life of p53 protein in normal mammary epithelial cells was ∼ 3 h, it was reduced to ∼ 15 min in all the E6-immortalized cells. These results demonstrate that the E6 genes of both high-risk and low-risk papilloma viruses immortalize human mammary epithelial cells and induce a marked degradation of p53 protein in vivo.

Original languageEnglish (US)
Pages (from-to)1847-1852
Number of pages6
JournalEMBO Journal
Volume12
Issue number5
StatePublished - Dec 1 1993

Fingerprint

Bovine papillomavirus 1
Human papillomavirus 6
Proteolysis
Breast
Genes
Epithelial Cells
Viruses
Degradation
Human papillomavirus 16
Papillomaviridae
Uterine Cervical Neoplasms
Proteins
Cells
Immunoprecipitation
Half-Life
Cell Line

Keywords

  • E6
  • Half-life
  • Human papillomavirus
  • Mammary epithelial cells
  • p53 protein

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Enhanced degradation of p53 protein in HPV-6 and BPV-1 E6-immortalized human mammary epithelial cells. / Band, Vimla; Dalal, Sorab; Delmolino, Laurie; Androphy, Elliot J.

In: EMBO Journal, Vol. 12, No. 5, 01.12.1993, p. 1847-1852.

Research output: Contribution to journalArticle

Band, V, Dalal, S, Delmolino, L & Androphy, EJ 1993, 'Enhanced degradation of p53 protein in HPV-6 and BPV-1 E6-immortalized human mammary epithelial cells', EMBO Journal, vol. 12, no. 5, pp. 1847-1852.
Band, Vimla ; Dalal, Sorab ; Delmolino, Laurie ; Androphy, Elliot J. / Enhanced degradation of p53 protein in HPV-6 and BPV-1 E6-immortalized human mammary epithelial cells. In: EMBO Journal. 1993 ; Vol. 12, No. 5. pp. 1847-1852.
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