Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells

Shantaram S Joshi, S. J. O'connor, D. D. Weisenburger, John G Sharp, H. M. Gharpure, K. W. Brunson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The highly malignant/metastatic murine large cell lymphoma cell line RAW117-H10 forms 100-200 times more liver metastatic tumors than its parental counterpart cell line RAW117-P. RAW117-H10 cells, but not the less malignant/metastatic parental cells, significantly inhibited the mitogen-induced proliferation of normal syngeneic Balb/c and allogeneic ICRC mouse spleen cells. Such an inhibition also occurred when mitomycin-C treated metastatic lymphoma cells were added 24 h after initiation of culture, indicating that no competition with mitogen binding sites on the lymphocytes was necssary for inhibition of proliferation. 'Antiproliferative' cell surface molecules were extracted non-cytolytically from the RAW117-H10 cells using butanol. The butanol extracts from the metastatic RAW117-H10 cells also inhibited the mitogen-induced proliferation and natural killer (NK) cell-mediated cytotoxicity of normal spleen cells. Our results indicate that these 'antiproliferative' cell surface molecules of metastatic murine RAW117-H10 lymphoma cells may have important role(s) in tumor-mediated host immunosuppression.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalClinical & Experimental Metastasis
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1991

Fingerprint

Lymphoma
Mitogens
Butanols
Spleen
Cell Line
Mitomycin
Natural Killer Cells
Immunosuppression
Neoplasms
Binding Sites
Lymphocytes
Liver

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells. / Joshi, Shantaram S; O'connor, S. J.; Weisenburger, D. D.; Sharp, John G; Gharpure, H. M.; Brunson, K. W.

In: Clinical & Experimental Metastasis, Vol. 9, No. 1, 01.01.1991, p. 27-37.

Research output: Contribution to journalArticle

Joshi, Shantaram S ; O'connor, S. J. ; Weisenburger, D. D. ; Sharp, John G ; Gharpure, H. M. ; Brunson, K. W. / Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells. In: Clinical & Experimental Metastasis. 1991 ; Vol. 9, No. 1. pp. 27-37.
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