Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes

Punniyakotti Elumalai, Jeong Muk Lim, Yool Jin Park, Min Cho, Patrick J Shea, Byung Taek Oh

Research output: Contribution to journalArticle

Abstract

A chemotrophic, aerobic bacterial strain, Bacillus subtilis B2, was used to produce amylase by submerged fermentation under different light sources. SDS-PAGE indicated that the 55 kDa enzyme belonged to the α-amylase group. B2 was incubated in basal media with 1% soluble starch (pH 7.0) under blue, green, red, and white light-emitting diodes (LEDs), and white fluorescent light. Fermentation under blue LEDs maximized amylase production (180.59 ± 1.6 U/mL at 24 h). Production at 48 h increased to 310.56 ± 1.6 U/mL with 5% glucose as a simple carbon source and to 300.51 ± 1.7 U/mL with 5% groundnut oil cake as an agricultural waste substrate. Activity and stability of the amylase were greatest at pH 7.0 and 45–55 °C. Na+, Ca2+, Mg2+, Co2+, Ba2+, and K+ increased activity, while Ni2+, Hg2+, Mn2+, Cu2+, Fe3+, and Zn2+ inhibited activity. EDTA, PMSF and DTNB reduced activity by 50% or more, while tetrafluoroethylene and 1,10-phenanthroline reduced activity by 30%. The amylase was highly tolerant of the surfactants, compatible with organic solvents, oxidizing agents and the reducing agents reduced activity. These properties suggest utility of amylase produced by B. subtilis B2 under blue LED-mediated fermentation for industrial applications.

Original languageEnglish (US)
JournalPreparative Biochemistry and Biotechnology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Bacilli
Amylases
Bacillus subtilis
Light emitting diodes
Light
Fermentation
Dithionitrobenzoic Acid
Agricultural wastes
Reducing Agents
Oxidants
Surface-Active Agents
Edetic Acid
Starch
Organic solvents
Industrial applications
Light sources
Polyacrylamide Gel Electrophoresis
Oils
Carbon
Glucose

Keywords

  • Amylase
  • B. subtilis
  • blue LEDs
  • metal ions
  • photoreceptor
  • surfactant

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry

Cite this

Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes. / Elumalai, Punniyakotti; Lim, Jeong Muk; Park, Yool Jin; Cho, Min; Shea, Patrick J; Oh, Byung Taek.

In: Preparative Biochemistry and Biotechnology, 01.01.2018.

Research output: Contribution to journalArticle

Elumalai, Punniyakotti ; Lim, Jeong Muk ; Park, Yool Jin ; Cho, Min ; Shea, Patrick J ; Oh, Byung Taek. / Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes. In: Preparative Biochemistry and Biotechnology. 2018.
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