Enhanced activity of carotid body chemoreceptors in rabbits with heart failure: Role of nitric oxide

Shu Yu Sun, W. Wang, Irving H Zucker, Harold D Schultz

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

An enhanced peripheral chemoreflex has been documented in patients with chronic heart failure (CHF). This study aimed to examine the characteristics of carotid body (CB) chemoreceptors in response to isocapnic hypoxia in a rabbit model of pacing-induced CHF and to evaluate the possible role that nitric oxide (NO) plays in the altered characteristics. The chemosensitive characteristics of the CB were evaluated by recording single-unit activity from the carotid sinus nerve in both an intact and a vascularly isolated preparation. It was found that the baseline discharge under normoxia (intact preparation: arterial PO2 90-95 Torr; isolated preparation: PO2 100-110 Torr) and the chemosensitivity in response to graded hypoxia (PO2 40-70 Torr) were enhanced in CHF vs. sham rabbits. These alterations were independent of the CB preparations (intact vs. isolated). NO synthase inhibition by N(ω)-nitro-L-arginine increased the baseline discharge and the chemosensitivity in the intact preparation, whereas L-arginine (10-5 M) inhibited the baseline discharge and the chemosensitivity in the isolated preparation in sham but not in CHF rabbits. S-nitroso-N-acetylpenicillamine, an NO donor, inhibited the baseline discharge and the chemosensitivity in both CB preparations in CHF rabbits but only in the isolated preparation in sham rabbits. The amount of NO produced in vitro by the CB under normoxia was less in CHF rabbits than in sham rabbits (P < 0.05). NO synthase-positive varicosities of nerve fibers within the CB were less in CHF rabbits than in sham rabbits (P < 0.05). These data indicate that an enhanced input from CB occurs in the rabbit model of pacing-induced CHF and that an impairment of NO production may contribute to this alteration.

Original languageEnglish (US)
Pages (from-to)1273-1282
Number of pages10
JournalJournal of Applied Physiology
Volume86
Issue number4
DOIs
StatePublished - Apr 1999

Fingerprint

Carotid Body
Nitric Oxide
Heart Failure
Rabbits
Nitric Oxide Synthase
Arginine
S-Nitroso-N-Acetylpenicillamine
Carotid Sinus
Nitric Oxide Donors
Nerve Fibers

Keywords

  • Carotid sinus nerve
  • Hypoxia
  • N(ω)-nitro-L-arginine
  • S- nitroso-N-acetyl-penicillamine
  • Single-unit activity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Enhanced activity of carotid body chemoreceptors in rabbits with heart failure : Role of nitric oxide. / Sun, Shu Yu; Wang, W.; Zucker, Irving H; Schultz, Harold D.

In: Journal of Applied Physiology, Vol. 86, No. 4, 04.1999, p. 1273-1282.

Research output: Contribution to journalArticle

@article{1d990b0ebdab4a6bb865eb934c7e8181,
title = "Enhanced activity of carotid body chemoreceptors in rabbits with heart failure: Role of nitric oxide",
abstract = "An enhanced peripheral chemoreflex has been documented in patients with chronic heart failure (CHF). This study aimed to examine the characteristics of carotid body (CB) chemoreceptors in response to isocapnic hypoxia in a rabbit model of pacing-induced CHF and to evaluate the possible role that nitric oxide (NO) plays in the altered characteristics. The chemosensitive characteristics of the CB were evaluated by recording single-unit activity from the carotid sinus nerve in both an intact and a vascularly isolated preparation. It was found that the baseline discharge under normoxia (intact preparation: arterial PO2 90-95 Torr; isolated preparation: PO2 100-110 Torr) and the chemosensitivity in response to graded hypoxia (PO2 40-70 Torr) were enhanced in CHF vs. sham rabbits. These alterations were independent of the CB preparations (intact vs. isolated). NO synthase inhibition by N(ω)-nitro-L-arginine increased the baseline discharge and the chemosensitivity in the intact preparation, whereas L-arginine (10-5 M) inhibited the baseline discharge and the chemosensitivity in the isolated preparation in sham but not in CHF rabbits. S-nitroso-N-acetylpenicillamine, an NO donor, inhibited the baseline discharge and the chemosensitivity in both CB preparations in CHF rabbits but only in the isolated preparation in sham rabbits. The amount of NO produced in vitro by the CB under normoxia was less in CHF rabbits than in sham rabbits (P < 0.05). NO synthase-positive varicosities of nerve fibers within the CB were less in CHF rabbits than in sham rabbits (P < 0.05). These data indicate that an enhanced input from CB occurs in the rabbit model of pacing-induced CHF and that an impairment of NO production may contribute to this alteration.",
keywords = "Carotid sinus nerve, Hypoxia, N(ω)-nitro-L-arginine, S- nitroso-N-acetyl-penicillamine, Single-unit activity",
author = "Sun, {Shu Yu} and W. Wang and Zucker, {Irving H} and Schultz, {Harold D}",
year = "1999",
month = "4",
doi = "10.1152/jappl.1999.86.4.1273",
language = "English (US)",
volume = "86",
pages = "1273--1282",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Enhanced activity of carotid body chemoreceptors in rabbits with heart failure

T2 - Role of nitric oxide

AU - Sun, Shu Yu

AU - Wang, W.

AU - Zucker, Irving H

AU - Schultz, Harold D

PY - 1999/4

Y1 - 1999/4

N2 - An enhanced peripheral chemoreflex has been documented in patients with chronic heart failure (CHF). This study aimed to examine the characteristics of carotid body (CB) chemoreceptors in response to isocapnic hypoxia in a rabbit model of pacing-induced CHF and to evaluate the possible role that nitric oxide (NO) plays in the altered characteristics. The chemosensitive characteristics of the CB were evaluated by recording single-unit activity from the carotid sinus nerve in both an intact and a vascularly isolated preparation. It was found that the baseline discharge under normoxia (intact preparation: arterial PO2 90-95 Torr; isolated preparation: PO2 100-110 Torr) and the chemosensitivity in response to graded hypoxia (PO2 40-70 Torr) were enhanced in CHF vs. sham rabbits. These alterations were independent of the CB preparations (intact vs. isolated). NO synthase inhibition by N(ω)-nitro-L-arginine increased the baseline discharge and the chemosensitivity in the intact preparation, whereas L-arginine (10-5 M) inhibited the baseline discharge and the chemosensitivity in the isolated preparation in sham but not in CHF rabbits. S-nitroso-N-acetylpenicillamine, an NO donor, inhibited the baseline discharge and the chemosensitivity in both CB preparations in CHF rabbits but only in the isolated preparation in sham rabbits. The amount of NO produced in vitro by the CB under normoxia was less in CHF rabbits than in sham rabbits (P < 0.05). NO synthase-positive varicosities of nerve fibers within the CB were less in CHF rabbits than in sham rabbits (P < 0.05). These data indicate that an enhanced input from CB occurs in the rabbit model of pacing-induced CHF and that an impairment of NO production may contribute to this alteration.

AB - An enhanced peripheral chemoreflex has been documented in patients with chronic heart failure (CHF). This study aimed to examine the characteristics of carotid body (CB) chemoreceptors in response to isocapnic hypoxia in a rabbit model of pacing-induced CHF and to evaluate the possible role that nitric oxide (NO) plays in the altered characteristics. The chemosensitive characteristics of the CB were evaluated by recording single-unit activity from the carotid sinus nerve in both an intact and a vascularly isolated preparation. It was found that the baseline discharge under normoxia (intact preparation: arterial PO2 90-95 Torr; isolated preparation: PO2 100-110 Torr) and the chemosensitivity in response to graded hypoxia (PO2 40-70 Torr) were enhanced in CHF vs. sham rabbits. These alterations were independent of the CB preparations (intact vs. isolated). NO synthase inhibition by N(ω)-nitro-L-arginine increased the baseline discharge and the chemosensitivity in the intact preparation, whereas L-arginine (10-5 M) inhibited the baseline discharge and the chemosensitivity in the isolated preparation in sham but not in CHF rabbits. S-nitroso-N-acetylpenicillamine, an NO donor, inhibited the baseline discharge and the chemosensitivity in both CB preparations in CHF rabbits but only in the isolated preparation in sham rabbits. The amount of NO produced in vitro by the CB under normoxia was less in CHF rabbits than in sham rabbits (P < 0.05). NO synthase-positive varicosities of nerve fibers within the CB were less in CHF rabbits than in sham rabbits (P < 0.05). These data indicate that an enhanced input from CB occurs in the rabbit model of pacing-induced CHF and that an impairment of NO production may contribute to this alteration.

KW - Carotid sinus nerve

KW - Hypoxia

KW - N(ω)-nitro-L-arginine

KW - S- nitroso-N-acetyl-penicillamine

KW - Single-unit activity

UR - http://www.scopus.com/inward/record.url?scp=0032960471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032960471&partnerID=8YFLogxK

U2 - 10.1152/jappl.1999.86.4.1273

DO - 10.1152/jappl.1999.86.4.1273

M3 - Article

C2 - 10194213

AN - SCOPUS:0032960471

VL - 86

SP - 1273

EP - 1282

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

IS - 4

ER -