Energetics, Ion, and Water Binding of the Unfolding of AA/UU Base Pair Stacks and UAU/UAU Base Triplet Stacks in RNA

Carolyn E. Carr, Irine Khutsishvili, Luis A Marky

Research output: Contribution to journalArticle

Abstract

Triplex formation occurs via interaction of a third strand with the major groove of double-stranded nucleic acid, through Hoogsteen hydrogen bonding. In this work, we use a combination of temperature-dependent UV spectroscopy and differential scanning calorimetry to determine complete thermodynamic profiles for the unfolding of polyadenylic acid (poly(rA))·polyuridylic acid (poly(rU)) (duplex) and poly(rA)·2poly(rU) (triplex). Our thermodynamic results are in good agreement with the much earlier work of Krakauer and Sturtevant using only UV melting techniques. The folding of these two helices yielded an uptake of ions, δnNa+ = 0.15 mol Na+/mol base pair (duplex) and 0.30 mol Na+/mole base triplet (triplex), which are consistent with their polymer behavior and the higher charge density parameter of triple helices. The osmotic stress technique yielded a release of structural water, δnW = 2 mol H2O/mol base pair (duplex unfolding into single strands) and an uptake of structural water, δnW = 2 mol H2O/mole base pair (triplex unfolding into duplex and a single strand). However, an overall release of electrostricted waters is obtained for the unfolding of both complexes from pressure perturbation calorimetric experiments. In total, the δV values obtained for the unfolding of triplex into duplex and a single strand correspond to an immobilization of two structural waters and a release of three electrostricted waters. The δV values obtained for the unfolding of duplex into two single strands correspond to the release of two structural waters and the immobilization of four electrostricted water molecules.

Original languageEnglish (US)
Pages (from-to)7057-7065
Number of pages9
JournalJournal of Physical Chemistry B
Volume122
Issue number28
DOIs
StatePublished - Jul 19 2018

Fingerprint

RNA
Base Pairing
Ions
strands
Water
Poly A
water
ions
Poly U
immobilization
Thermodynamics
Immobilization
helices
Poly A-U
thermodynamics
acids
Acids
Nucleic acids
Osmotic Pressure
Differential Scanning Calorimetry

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Energetics, Ion, and Water Binding of the Unfolding of AA/UU Base Pair Stacks and UAU/UAU Base Triplet Stacks in RNA. / Carr, Carolyn E.; Khutsishvili, Irine; Marky, Luis A.

In: Journal of Physical Chemistry B, Vol. 122, No. 28, 19.07.2018, p. 7057-7065.

Research output: Contribution to journalArticle

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