Endotoxin exposure and inflammation markers among agricultural workers in Colorado and Nebraska

James B. Burch, Erik Svendsen, Paul D. Siegel, Sara E. Wagner, Susanna G Von Essen, Thomas Keefe, John Mehaffy, Angelica Serrano Martinez, Mary Bradford, Laura Baker, Brian Cranmer, Rena Saito, John Tessari, Prinz Linda, Colene Andersen, Opal Christensen, Niels Koehncke, Stephen J. Reynolds

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The adverse respiratory effects of agricultural dust inhalation are mediated in part by endotoxin, a constituent of gram-negative bacterial cell walls. This study quantified personal work-shift exposures to inhalable dust, endotoxin, and its reactive 3-hydroxy fatty acid (3-OHFA) constituents among workers in grain elevators, cattle feedlots, dairies, and on corn farms. Exposures were compared with post-work-shift nasal lavage fluid inflammation markers and respiratory symptoms. Breathing-zone personal air monitoring was performed over one work shift to quantify inhalable dust (Institute of Medicine samplers), endotoxin (recombinant factor C [rFC] assay), and 3-OHFA (gas chromatography/mass spectrometry). Post-shift nasal lavage fluids were assayed for polymorphonuclear neutrophils (PMN), myeloperoxidase (MPO), interleukin 8 (IL-8), albumin, and eosinophilic cation protein (ECP) concentrations. The geometric mean (GSD) of endotoxin exposure (rFC assay) among the 125 male participants was 888 ± (6.5) EU/m3, and 93% exceeded the proposed exposure limit (50 EU/m3). Mean PMN, MPO, albumin, and ECP levels were two- to threefold higher among workers in the upper quartile of 3-OHFA exposure compared to the lowest exposure quartile. Even numbered 3-OHFA were most strongly associated with nasal inflammation. Symptom prevalence was not elevated among exposed workers, possibly due to endotoxin tolerance or a healthy worker effect in this population. This is the first study to evaluate the relationship between endotoxin's 3-OHFA constituents in agricultural dust and nasal airway inflammation. More research is needed to characterize the extent to which these agents contribute to respiratory disease among agricultural workers.

Original languageEnglish (US)
Pages (from-to)5-22
Number of pages18
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume73
Issue number1
DOIs
StatePublished - Jan 1 2010

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Endotoxins
Inflammation
Dust
Nasal Lavage Fluid
Agricultural Workers' Diseases
Nose
Peroxidase
Cations
Grain elevators
Albumins
Assays
Neutrophils
Healthy Worker Effect
Elevators and Escalators
Pulmonary diseases
Hydroxy Acids
National Academies of Science, Engineering, and Medicine (U.S.) Health and Medicine Division
Fluids
Dairies
Interleukin-8

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Endotoxin exposure and inflammation markers among agricultural workers in Colorado and Nebraska. / Burch, James B.; Svendsen, Erik; Siegel, Paul D.; Wagner, Sara E.; Von Essen, Susanna G; Keefe, Thomas; Mehaffy, John; Martinez, Angelica Serrano; Bradford, Mary; Baker, Laura; Cranmer, Brian; Saito, Rena; Tessari, John; Linda, Prinz; Andersen, Colene; Christensen, Opal; Koehncke, Niels; Reynolds, Stephen J.

In: Journal of Toxicology and Environmental Health - Part A: Current Issues, Vol. 73, No. 1, 01.01.2010, p. 5-22.

Research output: Contribution to journalArticle

Burch, JB, Svendsen, E, Siegel, PD, Wagner, SE, Von Essen, SG, Keefe, T, Mehaffy, J, Martinez, AS, Bradford, M, Baker, L, Cranmer, B, Saito, R, Tessari, J, Linda, P, Andersen, C, Christensen, O, Koehncke, N & Reynolds, SJ 2010, 'Endotoxin exposure and inflammation markers among agricultural workers in Colorado and Nebraska', Journal of Toxicology and Environmental Health - Part A: Current Issues, vol. 73, no. 1, pp. 5-22. https://doi.org/10.1080/15287390903248604
Burch, James B. ; Svendsen, Erik ; Siegel, Paul D. ; Wagner, Sara E. ; Von Essen, Susanna G ; Keefe, Thomas ; Mehaffy, John ; Martinez, Angelica Serrano ; Bradford, Mary ; Baker, Laura ; Cranmer, Brian ; Saito, Rena ; Tessari, John ; Linda, Prinz ; Andersen, Colene ; Christensen, Opal ; Koehncke, Niels ; Reynolds, Stephen J. / Endotoxin exposure and inflammation markers among agricultural workers in Colorado and Nebraska. In: Journal of Toxicology and Environmental Health - Part A: Current Issues. 2010 ; Vol. 73, No. 1. pp. 5-22.
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