Endothelium-dependent responses of cerebral arterioles to adenosine 5'-diphosphate

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Abstract

The goal of this study was to determine the role of endothelium-derived relaxing factor (EDRF) in dilatation of cerebral arterioles in response to adenosine 5'-diphosphate (ADP). Using intravital microscopy, we measured the diameters of pial arterioles in vivo in rats during superfusion with ADP, acetylcholine and nitroglycerin before and during topical application of inhibitors of nitric oxide synthesis (N(G)-monomethyl-L-arginine; L-NMMA and N(w)-nitro-L-arginine; L-NNA). Prior to suffusion with L-NMMA and L-NNA, ADP (10 and 100 μM), acetylcholine (0.1 and 1.0 μM), and nitroglycerin (1.0 and 10 μM) produced dose-related increases in the diameter of pial arterioles. Following application of L-NMMA (1.0 and 10 μM) and L-NNA (0.1 and 1.0 μM), dilatation of cerebral arterioles in response to ADP was significantly inhibited. In addition, L-NMMA and L-NNA significantly inhibited dilatation of cerebral arterioles in response to acetylcholine, but did not alter vasodilatation in response to nitroglycerin. Thus, our findings suggest that ADP dilates cerebral arterioles via the production of nitric oxide, or a nitric-oxide-containing compound.

Original languageEnglish (US)
Pages (from-to)353-358
Number of pages6
JournalJournal of Vascular Research
Volume29
Issue number5
DOIs
StatePublished - Jan 1 1992

Fingerprint

Arterioles
Adenosine Diphosphate
Endothelium
omega-N-Methylarginine
Nitroglycerin
Acetylcholine
Dilatation
Nitric Oxide
Arginine
Endothelium-Dependent Relaxing Factors
Vasodilation

Keywords

  • ADP
  • EDRF
  • acetylcholine
  • brain
  • microcirculation
  • nitric oxide
  • nitroglycerin
  • pial arterioles
  • rats

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Endothelium-dependent responses of cerebral arterioles to adenosine 5'-diphosphate. / Mayhan, W. G.

In: Journal of Vascular Research, Vol. 29, No. 5, 01.01.1992, p. 353-358.

Research output: Contribution to journalArticle

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