Abstract
The goal of this study was to determine the role of endothelium-derived relaxing factor (EDRF) in dilatation of cerebral arterioles in response to adenosine 5'-diphosphate (ADP). Using intravital microscopy, we measured the diameters of pial arterioles in vivo in rats during superfusion with ADP, acetylcholine and nitroglycerin before and during topical application of inhibitors of nitric oxide synthesis (N(G)-monomethyl-L-arginine; L-NMMA and N(w)-nitro-L-arginine; L-NNA). Prior to suffusion with L-NMMA and L-NNA, ADP (10 and 100 μM), acetylcholine (0.1 and 1.0 μM), and nitroglycerin (1.0 and 10 μM) produced dose-related increases in the diameter of pial arterioles. Following application of L-NMMA (1.0 and 10 μM) and L-NNA (0.1 and 1.0 μM), dilatation of cerebral arterioles in response to ADP was significantly inhibited. In addition, L-NMMA and L-NNA significantly inhibited dilatation of cerebral arterioles in response to acetylcholine, but did not alter vasodilatation in response to nitroglycerin. Thus, our findings suggest that ADP dilates cerebral arterioles via the production of nitric oxide, or a nitric-oxide-containing compound.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 353-358 |
| Number of pages | 6 |
| Journal | Journal of Vascular Research |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1992 |
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Keywords
- ADP
- EDRF
- acetylcholine
- brain
- microcirculation
- nitric oxide
- nitroglycerin
- pial arterioles
- rats
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
Endothelium-dependent responses of cerebral arterioles to adenosine 5'-diphosphate. / Mayhan, W. G.
In: Journal of Vascular Research, Vol. 29, No. 5, 01.01.1992, p. 353-358.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endothelium-dependent responses of cerebral arterioles to adenosine 5'-diphosphate
AU - Mayhan, W. G.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - The goal of this study was to determine the role of endothelium-derived relaxing factor (EDRF) in dilatation of cerebral arterioles in response to adenosine 5'-diphosphate (ADP). Using intravital microscopy, we measured the diameters of pial arterioles in vivo in rats during superfusion with ADP, acetylcholine and nitroglycerin before and during topical application of inhibitors of nitric oxide synthesis (N(G)-monomethyl-L-arginine; L-NMMA and N(w)-nitro-L-arginine; L-NNA). Prior to suffusion with L-NMMA and L-NNA, ADP (10 and 100 μM), acetylcholine (0.1 and 1.0 μM), and nitroglycerin (1.0 and 10 μM) produced dose-related increases in the diameter of pial arterioles. Following application of L-NMMA (1.0 and 10 μM) and L-NNA (0.1 and 1.0 μM), dilatation of cerebral arterioles in response to ADP was significantly inhibited. In addition, L-NMMA and L-NNA significantly inhibited dilatation of cerebral arterioles in response to acetylcholine, but did not alter vasodilatation in response to nitroglycerin. Thus, our findings suggest that ADP dilates cerebral arterioles via the production of nitric oxide, or a nitric-oxide-containing compound.
AB - The goal of this study was to determine the role of endothelium-derived relaxing factor (EDRF) in dilatation of cerebral arterioles in response to adenosine 5'-diphosphate (ADP). Using intravital microscopy, we measured the diameters of pial arterioles in vivo in rats during superfusion with ADP, acetylcholine and nitroglycerin before and during topical application of inhibitors of nitric oxide synthesis (N(G)-monomethyl-L-arginine; L-NMMA and N(w)-nitro-L-arginine; L-NNA). Prior to suffusion with L-NMMA and L-NNA, ADP (10 and 100 μM), acetylcholine (0.1 and 1.0 μM), and nitroglycerin (1.0 and 10 μM) produced dose-related increases in the diameter of pial arterioles. Following application of L-NMMA (1.0 and 10 μM) and L-NNA (0.1 and 1.0 μM), dilatation of cerebral arterioles in response to ADP was significantly inhibited. In addition, L-NMMA and L-NNA significantly inhibited dilatation of cerebral arterioles in response to acetylcholine, but did not alter vasodilatation in response to nitroglycerin. Thus, our findings suggest that ADP dilates cerebral arterioles via the production of nitric oxide, or a nitric-oxide-containing compound.
KW - ADP
KW - EDRF
KW - acetylcholine
KW - brain
KW - microcirculation
KW - nitric oxide
KW - nitroglycerin
KW - pial arterioles
KW - rats
UR - http://www.scopus.com/inward/record.url?scp=0026478732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026478732&partnerID=8YFLogxK
U2 - 10.1159/000158951
DO - 10.1159/000158951
M3 - Article
C2 - 1420730
AN - SCOPUS:0026478732
VL - 29
SP - 353
EP - 358
JO - Journal of Vascular Research
JF - Journal of Vascular Research
SN - 1018-1172
IS - 5
ER -