Endothelial microparticles induce inflammation in acute lung injury

Keely L Buesing, John C. Densmore, Sushma Kaul, Kirkwood A. Pritchard, Jason A. Jarzembowski, David M. Gourlay, Keith T. Oldham

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Previously, we have shown that endothelial microparticles (EMPs) injected into mice induce acute lung injury (ALI) [1]. In this study, we hypothesize that EMPs induce ALI by initiating cytokine release in the lung, leading to recruitment and activation of neutrophils. Materials and methods: C57BL/6J male mice (8-10 wk old) were intravenously injected with EMPs (200,000/mL), LPS (2 mg/kg), or both. Bronchoalveolar lavage (BAL) and serum levels of IL-1β and TNF-α were analyzed by enzyme-linked immunoassay (ELISA). Morphometric analysis was performed on H and E stained lung sections. Myeloperoxidase (MPO) levels were determined via an enzymatic assay and immunofluorescence of stained sections. Results: EMPs led to significantly increased pulmonary and systemic IL-1β and TNF-α levels, which correlated with increased neutrophil recruitment to the lung. MPO levels in the lungs were increased significantly following injection of EMPs or LPS, compared to PBS. In mice treated with EMPs and LPS either simultaneously or successively, the cytokine and MPO levels were significantly increased over that of either treatment alone. Conclusion: EMPs contribute to lung injury through the initiation of a cytokine cascade that increases recruitment of neutrophils and subsequent release of MPO. Furthermore, treatment of mice with both EMPs and LPS induced greater lung injury than either treatment alone, suggesting that EMPs prime the lung for increased injury by other pathogens. Therapies aimed at reducing or blocking EMPs may be a useful strategy for attenuating lung injury.

Original languageEnglish (US)
Pages (from-to)32-39
Number of pages8
JournalJournal of Surgical Research
Volume166
Issue number1
DOIs
StatePublished - Mar 1 2011

Fingerprint

Acute Lung Injury
Lung Injury
Peroxidase
Neutrophil Infiltration
Inflammation
Lung
Cytokines
Interleukin-1
Neutrophil Activation
Enzyme Assays
Bronchoalveolar Lavage
Therapeutics
Immunoenzyme Techniques
Fluorescent Antibody Technique
Injections
Serum

Keywords

  • IL-1β
  • TNF-α
  • endothelial microparticles
  • lung injury
  • myeloperoxidase

ASJC Scopus subject areas

  • Surgery

Cite this

Buesing, K. L., Densmore, J. C., Kaul, S., Pritchard, K. A., Jarzembowski, J. A., Gourlay, D. M., & Oldham, K. T. (2011). Endothelial microparticles induce inflammation in acute lung injury. Journal of Surgical Research, 166(1), 32-39. https://doi.org/10.1016/j.jss.2010.05.036

Endothelial microparticles induce inflammation in acute lung injury. / Buesing, Keely L; Densmore, John C.; Kaul, Sushma; Pritchard, Kirkwood A.; Jarzembowski, Jason A.; Gourlay, David M.; Oldham, Keith T.

In: Journal of Surgical Research, Vol. 166, No. 1, 01.03.2011, p. 32-39.

Research output: Contribution to journalArticle

Buesing, KL, Densmore, JC, Kaul, S, Pritchard, KA, Jarzembowski, JA, Gourlay, DM & Oldham, KT 2011, 'Endothelial microparticles induce inflammation in acute lung injury', Journal of Surgical Research, vol. 166, no. 1, pp. 32-39. https://doi.org/10.1016/j.jss.2010.05.036
Buesing KL, Densmore JC, Kaul S, Pritchard KA, Jarzembowski JA, Gourlay DM et al. Endothelial microparticles induce inflammation in acute lung injury. Journal of Surgical Research. 2011 Mar 1;166(1):32-39. https://doi.org/10.1016/j.jss.2010.05.036
Buesing, Keely L ; Densmore, John C. ; Kaul, Sushma ; Pritchard, Kirkwood A. ; Jarzembowski, Jason A. ; Gourlay, David M. ; Oldham, Keith T. / Endothelial microparticles induce inflammation in acute lung injury. In: Journal of Surgical Research. 2011 ; Vol. 166, No. 1. pp. 32-39.
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