Endogenous low-level expression of the coxsackievirus and adenovirus receptor enables coxsackievirus B3 infection of RD cells

Steven D Carson, Kyung Soo Kim, Samuel Jay Pirruccello, Steven Tracy, Nora M. Chapman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cells in which the appropriate viral receptor cannot be detected may paradoxically act as a host to the virus. For example, RD cells are often considered to be non-permissive for infection with coxsackievirus and adenovirus receptor (CAR)-dependent group B coxsackieviruses (CVB), insofar as inoculated cell monolayers show little or no cytopathic effect (CPE) and immunohistological assays for CAR have been consistently negative. Supernatants recovered from RD cells exposed to CVB, however, contained more virus than was added in the initial inoculum, indicating that productive virus replication occurred in the monolayer. When infected with a recombinant CVB type 3 (CVB3) chimeric strain expressing S-Tag within the viral polyprotein, 4-11 % of RD cells expressed S-Tag over 48 h. CAR mRNA was detected in RD cells by RT-PCR, and CAR protein was detected on Western blots of RD lysates; both were detected at much lower levels than in HeLa cells. Receptor blockade by an anti-CAR antibody confirmed that CVB3 infection of RD cells was mediated by CAR. These results show that some RD cells in the culture population express CAR and can thereby be infected by CVB, which explains the replication of CAR-dependent CVB in cell types that show little or no CPE and in which CAR has not previously been detected. Cells within cultures of cell types that have been considered non-permissive may express receptor transiently, leading to persistent replication of virus within the cultured population.

Original languageEnglish (US)
Pages (from-to)3031-3038
Number of pages8
JournalJournal of General Virology
Volume88
Issue number11
DOIs
StatePublished - Nov 1 2007

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Coxsackievirus Infections
Enterovirus
Virus Replication
Cell Culture Techniques
Viruses
Human Enterovirus B
adenovirus receptor
Polyproteins
HeLa Cells
Population
Western Blotting

ASJC Scopus subject areas

  • Virology

Cite this

Endogenous low-level expression of the coxsackievirus and adenovirus receptor enables coxsackievirus B3 infection of RD cells. / Carson, Steven D; Kim, Kyung Soo; Pirruccello, Samuel Jay; Tracy, Steven; Chapman, Nora M.

In: Journal of General Virology, Vol. 88, No. 11, 01.11.2007, p. 3031-3038.

Research output: Contribution to journalArticle

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