Endogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization

T. Watanabe, Y. Kawano, S. Kanamaru, T. Onishi, S. Kaneko, Y. Wakata, R. Nakagawa, A. Makimoto, Y. Kuroda, Y. Takaue, James E Talmadge

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Abstract

The relationship between stem cell mobilization with granulocyte colony- stimulating factor (G-CSF) and the endogenous production of interleukin-8 (IL-8), macrophage inflammatory protein-1α (MIP-1α), tumor necrosis factor- α (TNF-α), and interferon-γ (IFN-γ) was studied in normal donors for allogeneic peripheral blood stem cell (PBSC) transplantation. G-CSF was administered to 20 normal donors at a close of 10 μg/kg/d for 5 days with aphereses on clays 5 and 6 of G-CSF treatment. Cytokine serum levels were measured using an enzyme-linked immunosorbent assay (ELISA) before and during G-CSF treatment. Before treatment, the average level of IL-8 was 7.1 pg/mL, increasing to 207.0 pg/mL on day 5 and 189.1 pg/mL on day 6. Serum IL-8 levels correlated CD34+ cell numbers (P = .0151 and P = .0005 on days 5 and 6, respectively) and colony-forming unit-granulocyte-macrophage (CFU-GM) numbers (P = .0019 and P = .0010 on days 5 and 6, respectively). Furthermore, preapheresis serum IL-8 levels correlated with the yield of CD34+ cells (P = .0027). In contrast, before treatment, the average levels of MIP-1α, TNF- α, and IFN-γ were 70.1, 4.03, and 3.84 pg/mL, respectively, and no significant changes in the levels of these cytokines were observed during G- CSF treatment. These studies suggest that IL-8 production may be critical to G-CSF-induced stem cell mobilization, although the underlying mechanism could not be clarified.

Original languageEnglish (US)
Pages (from-to)1157-1163
Number of pages7
JournalBlood
Volume93
Issue number4
StatePublished - Feb 15 1999

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Hematopoietic Stem Cell Mobilization
Granulocyte Colony-Stimulating Factor
Stem cells
Interleukin-8
Blood
Macrophage Inflammatory Proteins
Interferons
Tumor Necrosis Factor-alpha
Serum
Cytokines
Therapeutics
Peripheral Blood Stem Cell Transplantation
Immunosorbents
Granulocyte-Macrophage Progenitor Cells
Blood Component Removal
Macrophages
Peripheral Blood Stem Cells
Assays
Cell Count
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Watanabe, T., Kawano, Y., Kanamaru, S., Onishi, T., Kaneko, S., Wakata, Y., ... Talmadge, J. E. (1999). Endogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization. Blood, 93(4), 1157-1163.

Endogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization. / Watanabe, T.; Kawano, Y.; Kanamaru, S.; Onishi, T.; Kaneko, S.; Wakata, Y.; Nakagawa, R.; Makimoto, A.; Kuroda, Y.; Takaue, Y.; Talmadge, James E.

In: Blood, Vol. 93, No. 4, 15.02.1999, p. 1157-1163.

Research output: Contribution to journalArticle

Watanabe, T, Kawano, Y, Kanamaru, S, Onishi, T, Kaneko, S, Wakata, Y, Nakagawa, R, Makimoto, A, Kuroda, Y, Takaue, Y & Talmadge, JE 1999, 'Endogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization', Blood, vol. 93, no. 4, pp. 1157-1163.
Watanabe T, Kawano Y, Kanamaru S, Onishi T, Kaneko S, Wakata Y et al. Endogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization. Blood. 1999 Feb 15;93(4):1157-1163.
Watanabe, T. ; Kawano, Y. ; Kanamaru, S. ; Onishi, T. ; Kaneko, S. ; Wakata, Y. ; Nakagawa, R. ; Makimoto, A. ; Kuroda, Y. ; Takaue, Y. ; Talmadge, James E. / Endogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization. In: Blood. 1999 ; Vol. 93, No. 4. pp. 1157-1163.
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