Endogenous butyrylcholinesterase in SV40 transformed cell lines: COS-1, COS-7, MRC-5 SV40, and WI-38 VA13

M. Kris, O. Jbilo, C. F. Bartels, P. Masson, S. Rhode, Oksana Lockridge

Research output: Contribution to journalArticle

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Abstract

Comparison of proteins expressed by SV40 transformed cell lines and untransformed cell lines is of interest because SV40 transformed cells are immortal, whereas untransformed cells senesce after about 50 doublings. In MRC-5 SV40 cells, only seven proteins have previously been reported to shift from undetectable to detectable after transformation by SV40 virus. We report that butyrylcholinesterase is an 8th protein in this category. Butyrylcholinesterase activity in transformed MRC-5 SV40 cells increased at least 150-fold over its undetectable level in MRC-5 parental cells. Other SV40 transformed cell lines, including COS-1, COS-7, and WI-38 VA13, also expressed endogenous butyrylcholinesterase, whereas the parental, untransformed cell lines, CV-1 and WI-38, had no detectable butyrylcholinesterase activity or mRNA. Infection of CV-1 cells by SV40 virus did not result in expression of butyrylcholinesterase, showing that the butyrylcholinesterase promoter was not activated by the large T antigen of SV40. We conclude that butyrylcholinesterase expression resulted from events related to cell immortalization and did not result from activation by the large T antigen.

Original languageEnglish (US)
Pages (from-to)680-689
Number of pages10
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume30
Issue number10
DOIs
StatePublished - Oct 1994

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Butyrylcholinesterase
Transformed Cell Line
Cells
Simian virus 40
Viral Tumor Antigens
Viruses
Cell Line
Proteins
Chemical activation
Messenger RNA
Infection

Keywords

  • SV40 transformed
  • butyrylcholinesterase
  • cholinesterase
  • immortalization
  • large T antigen

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Endogenous butyrylcholinesterase in SV40 transformed cell lines : COS-1, COS-7, MRC-5 SV40, and WI-38 VA13. / Kris, M.; Jbilo, O.; Bartels, C. F.; Masson, P.; Rhode, S.; Lockridge, Oksana.

In: In Vitro Cellular & Developmental Biology - Animal, Vol. 30, No. 10, 10.1994, p. 680-689.

Research output: Contribution to journalArticle

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