Endemic necrotizing enterocolitis: Lack of association with a specific infectious agent

Sunil Gupta, J. Glenn Morris, Pinaki Panigrahi, James P. Nataro, Roger I. Glass, Ira H. Gewolb

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We conducted a comprehensive analysis of bacterial, parasitic and viral agents present in stool samples of 23 necrotizing enterocolitis cases and 23 matched and 10 random controls. Enterococcus spp. Staphylococcus epidermidis, and Escherichia coli were the most common aerobic bacterial species isolated. Astrovirus was identified in a stool sample from one control. Eight infants were bacteremic; in 7 of 8 the same organism was also present in the stool. No one bacterial species or strain (as identified by plasmid profile analysis) was associated with occurrence of illness. Plasmid analysis further suggested that each infant was colonized with his or her own distinctive aerobic bacterial flora. With the exception of isolates from two control patients which hybridized with a probe fordiffuse adherence, no diarrheagenic E. coli was identified. Five (45%) of 11 caseinfants were colonized with coagulase-negative staphylococci (all S. epidermidis) that produced delta-hemolysin in vitro, as compared with 13 (87%) of 15 control infants. Necrotizing enterocolitis was not associated with an increased ability to ferment carbohydrate, as measured by in uitro beta-galactosidase activity. Our data do not support the hypothesis that endemic necrotizing enterocolitis in our institution is caused by a single infectious agent, nor was there evidence that previously proposed virulence mechanisms such as production of delta-hemolysin or increased in vitro carbohydrate fermentation play a critical role in disease occurrence.

Original languageEnglish (US)
Pages (from-to)728-733
Number of pages6
JournalPediatric Infectious Disease Journal
Volume13
Issue number8
DOIs
StatePublished - Aug 1994

Fingerprint

Necrotizing Enterocolitis
Staphylococcus epidermidis
Plasmids
Carbohydrates
Escherichia coli
Coagulase
Enterococcus
beta-Galactosidase
Staphylococcus
Fermentation
Virulence
In Vitro Techniques
Staphylococcus aureus delta hemolysin protein

Keywords

  • Infectious agent
  • Necrotizing enterocolitis (endemic)

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Gupta, S., Morris, J. G., Panigrahi, P., Nataro, J. P., Glass, R. I., & Gewolb, I. H. (1994). Endemic necrotizing enterocolitis: Lack of association with a specific infectious agent. Pediatric Infectious Disease Journal, 13(8), 728-733. https://doi.org/10.1097/00006454-199408000-00010

Endemic necrotizing enterocolitis : Lack of association with a specific infectious agent. / Gupta, Sunil; Morris, J. Glenn; Panigrahi, Pinaki; Nataro, James P.; Glass, Roger I.; Gewolb, Ira H.

In: Pediatric Infectious Disease Journal, Vol. 13, No. 8, 08.1994, p. 728-733.

Research output: Contribution to journalArticle

Gupta, Sunil ; Morris, J. Glenn ; Panigrahi, Pinaki ; Nataro, James P. ; Glass, Roger I. ; Gewolb, Ira H. / Endemic necrotizing enterocolitis : Lack of association with a specific infectious agent. In: Pediatric Infectious Disease Journal. 1994 ; Vol. 13, No. 8. pp. 728-733.
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