Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor cone-jet mode

Jingwei Xie, Chi Hwa Wang

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Solvent extraction (or evaporation from a W1/O/W 2-dispersion), coacervation, and spray drying methods are commonly employed to encapsulate protein drugs in polymeric microparticles for sustained delivery applications. To overcome the limitations of these methods, a novel etectrospray method was developed to encapsulate a model protein drug - bovine serum albumin (BSA) in biodegradable polymeric microparticles and examine the feasibility of the process in not denaturing the protein. Microparticles of approximately 20 μm diameter with corrugated surfaces and smooth surfaces were observed by scanning electron microscope. Confocal laser scanning microscope images showed that BSA was distributed evenly in microparticles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was employed to investigate the protein integrity of BSA released from the polymer matrix after 38 days. No protein degradation was observed during the 38 days release. The secondary structure of released BSA was characterized by Fourier transform infrared (FTIR) and circular dichroism (CD), which suggested that the released BSA was almost identical to native BSA. The encapsulation efficiency could reach 76% by adjusting the amount of the additive Pluronic F127 and processing parameters. The release profile could be tailored by the fabrication process and the sustained release of BSA could endure for more than 1 month. More than 80% of the bioactivity of BSA (evaluated by BSA ELISA kit) could be maintained after releasing from polymer matrix. Findings of the present study demonstrate that this novel electrospray method is a promising approach to encapsulate bioactive materials such as proteins, enzymes, antibiotics, and DNA fragments in biodegradable polymeric particles.

Original languageEnglish (US)
Pages (from-to)1278-1290
Number of pages13
JournalBiotechnology and Bioengineering
Volume97
Issue number5
DOIs
StatePublished - Aug 1 2007

Fingerprint

Bovine Serum Albumin
Encapsulation
Cones
Proteins
Polymer matrix
UCON 50-HB-5100
Scanning
Spray drying
Polymers
Dichroism
Sodium dodecyl sulfate
Solvent extraction
Antibiotics
Bioactivity
Polyacrylates
Electrophoresis
Poloxamer
Fourier transforms
Evaporation
Microscopes

Keywords

  • Cone-jet mode
  • Electrospray
  • Microparticles
  • PLGA
  • Protein microencapsulation

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor cone-jet mode. / Xie, Jingwei; Wang, Chi Hwa.

In: Biotechnology and Bioengineering, Vol. 97, No. 5, 01.08.2007, p. 1278-1290.

Research output: Contribution to journalArticle

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