Elucidating causes of Diporeia decline in the great lakes via metabolomics: Physiological responses after exposure to different stressors

Suman Maity, Amber Jannasch, Jiri Adamec, James M. Watkins, Thomas Nalepa, Tomas O. Höök, Maria S. Sepúlveda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The benthic macroinvertebrate Diporeia spp. have been extirpated from many areas of the Laurentian Great Lakes, but the mechanisms underlying such declines are not fully understood. Diporeia declines coinciding with the invasion of exotic dreissenid mussels (zebra and quagga) have led to the hypothesis that Diporeia declines are a result of decreased food availability from increasing competition with dreissenids for diatoms. There is additional evidence that Diporeia are negatively affected when in close proximity to dreissenids, probably because of exposure to toxins present in the mussels' pseudofeces. Diporeia are also known to be sensitive to anthropogenic contaminants (such as polychlorinated biphenyls [PCBs]) present in Great Lakes sediments. To better understand the physiological responses of Diporeia to diverse stressors, we conducted three 28-d experiments evaluating changes in the metabolomes of Diporeia (1) fed diatoms (Cyclotella meneghiniana) versus starved, (2) exposed (from Lake Michigan and Cayuga Lake) to quagga mussels (Dreissena bugensis), and (3) exposed to sediments contaminated with PCBs. The metabolomes of samples were examined using both two-dimensional gas and liquid chromatography coupled with mass spectrometry. Each stressor elicited a unique metabolome response characterized by enhanced citric acid cycle, fatty acid biosynthesis, and protein metabolism in diatom-fed Diporeia; impaired glycolysis, protein catabolism, and folate metabolism in Diporeia from LakeMichigan irrespective of quagga mussel exposure, suggesting lakespecific adaptation mechanisms; and altered cysteine and phospholipid metabolism during PCB exposure. Subsequent comparisons of these stressor-specific metabolic responses with metabolomes of a feral Diporeia population would help identify stressors affecting Diporeia populations throughout the Great Lakes.

Original languageEnglish (US)
Pages (from-to)213-223
Number of pages11
JournalPhysiological and Biochemical Zoology
Volume86
Issue number2
DOIs
StatePublished - Feb 1 2013

Fingerprint

Dreissena bugensis
metabolome
Metabolomics
metabolomics
Lakes
physiological response
Metabolome
Bacillariophyceae
polychlorinated biphenyls
Great Lakes
Diatoms
Bivalvia
Polychlorinated Biphenyls
Dreissena
lakes
Metabolism
protein metabolism
Cyclotella meneghiniana
Sediments
sediments

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Animal Science and Zoology

Cite this

Elucidating causes of Diporeia decline in the great lakes via metabolomics : Physiological responses after exposure to different stressors. / Maity, Suman; Jannasch, Amber; Adamec, Jiri; Watkins, James M.; Nalepa, Thomas; Höök, Tomas O.; Sepúlveda, Maria S.

In: Physiological and Biochemical Zoology, Vol. 86, No. 2, 01.02.2013, p. 213-223.

Research output: Contribution to journalArticle

Maity, Suman ; Jannasch, Amber ; Adamec, Jiri ; Watkins, James M. ; Nalepa, Thomas ; Höök, Tomas O. ; Sepúlveda, Maria S. / Elucidating causes of Diporeia decline in the great lakes via metabolomics : Physiological responses after exposure to different stressors. In: Physiological and Biochemical Zoology. 2013 ; Vol. 86, No. 2. pp. 213-223.
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