Elevated levels of DNA strand breaks induced by a base analog in the human cell line with the P32T ITPA variant

Irina S.R. Waisertreiger, Miriam R. Menezes, James Randazzo, Youri I. Pavlov

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Base analogs are powerful antimetabolites and dangerous mutagens generated endogenously by oxidative stress, inflammation, and aberrant nucleotide biosynthesis. Human inosine triphosphate pyrophosphatase (ITPA) hydrolyzes triphosphates of noncanonical purine bases (i.e., ITP, dITP, XTP, dXTP, or their mimic: 6-hydroxyaminopurine (HAP) deoxynucleoside triphosphate) and thus regulates nucleotide pools and protects cells from DNA damage. We demonstrate that the model purine base analog HAP induces DNA breaks in human cells and leads to elevation of levels of ITPA. A human polymorphic allele of the ITPA, 94C- > A encodes for the enzyme with a P32T amino-acid change and leads to accumulation of nonhydrolyzed ITP. The polymorphism has been associated with adverse reaction to purine base-analog drugs. The level of both spontaneous and HAP-induced DNA breaks is elevated in the cell line with the ITPA P32T variant. The results suggested that human ITPA plays a pivotal role in the protection of DNA from noncanonical purine base analogs.

Original languageEnglish (US)
Article number872180
JournalJournal of Nucleic Acids
Volume2010
DOIs
StatePublished - Dec 1 2010

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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