Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells

Marie Classon, Marie Henriksson, Janos Sümegi, George Klein, Marie Louise Hammaskjöld

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Abstract

The v-myc oncogene can induce tumours in haematopoietic, mesenchymal and epithelial tissues1. The corresponding c-myc proto-oncogene can contribute to the genesis and/or the progression of an equally wide variety of tumours when activated by retroviral insertions, chromosomal translocations or gene amplification2. The c-myc gene product is a DNA-binding, nuclear phosphoprotein that is involved in the control of cell proliferation and possibly in DNA synthesis2. The replication of Simian virus 40 (SV40) is a useful model system to study eukaryotic DNA replication as the virus relies almost entirely on cellular DNA replication apparatus. The SV40-based vector, pSVEpR4, replicates poorly in the human BJAB lymphoma line and in most human cells, but replicates well in Burkitt lymphoma lines, which have fused immunoglobulin and c-myc genes, resulting in high c-myc expression. Cotransfection of the BJAB cells with a c-myc-expressing construct (pI4-P6) increased the replication of pSVEpR4 tenfold. Our findings indicate that overexpression of the c-myc gene product allows the replication of SV40 in human lymphoma cells, suggesting that c-myc is involved in the control of replication.

Original languageEnglish (US)
Pages (from-to)272-274
Number of pages3
JournalNature
Volume330
Issue number6145
DOIs
StatePublished - Jan 1 1987

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myc Genes
Simian virus 40
Lymphoma
DNA
DNA Replication
Genetic Translocation
Burkitt Lymphoma
Phosphoproteins
Immunoglobulins
Neoplasms
Cell Proliferation
Viruses
Genes

ASJC Scopus subject areas

  • General

Cite this

Classon, M., Henriksson, M., Sümegi, J., Klein, G., & Hammaskjöld, M. L. (1987). Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells. Nature, 330(6145), 272-274. https://doi.org/10.1038/330272a0

Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells. / Classon, Marie; Henriksson, Marie; Sümegi, Janos; Klein, George; Hammaskjöld, Marie Louise.

In: Nature, Vol. 330, No. 6145, 01.01.1987, p. 272-274.

Research output: Contribution to journalArticle

Classon, M, Henriksson, M, Sümegi, J, Klein, G & Hammaskjöld, ML 1987, 'Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells', Nature, vol. 330, no. 6145, pp. 272-274. https://doi.org/10.1038/330272a0
Classon M, Henriksson M, Sümegi J, Klein G, Hammaskjöld ML. Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells. Nature. 1987 Jan 1;330(6145):272-274. https://doi.org/10.1038/330272a0
Classon, Marie ; Henriksson, Marie ; Sümegi, Janos ; Klein, George ; Hammaskjöld, Marie Louise. / Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells. In: Nature. 1987 ; Vol. 330, No. 6145. pp. 272-274.
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