Electrospun ultrafine fibrous wheat glutenin scaffolds with three-dimensionally random organization and water stability for soft tissue engineering

Helan Xu, Shaobo Cai, Alexander Sellers, Yiqi Yang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Wheat glutenin, the highly crosslinked protein from wheat, was electrospun into scaffolds with ultrafine fibers oriented randomly and evenly in three dimensions to simulate native extracellular matrices of soft tissues. The scaffolds were intrinsically water-stable without using any external crosslinkers and could support proliferation and differentiation of adipose-derived mesenchymal stem cells for soft tissue engineering. Regeneration of soft tissue favored water-stable fibrous protein scaffolds with three-dimensional arrangement and large volumes, which could be difficult to obtain via electrospinning. Wheat glutenin is an intrinsically water-stable protein due to the 2% cysteine in its amino acid composition. In this research, the disulfide crosslinks in wheat glutenin were cleaved while the backbones were preserved. The treated wheat glutenin was dissolved in aqueous solvent with an anionic surfactant and then electrospun into bulky scaffolds composed of ultrafine fibers oriented randomly in three dimensions. The scaffolds could maintain their fibrous structures after incubated in PBS for up to 35 days. In vitro study indicated that the three-dimensional wheat glutenin scaffolds well supported uniform distribution and adipogenic differentiation of adipose derived mesenchymal stem cells.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalJournal of Biotechnology
Volume184
DOIs
StatePublished - Aug 20 2014

Fingerprint

Tissue Engineering
Tissue engineering
Scaffolds
Triticum
Water
Scaffolds (biology)
Stem cells
Proteins
Tissue
Mesenchymal Stromal Cells
Fibers
Anionic surfactants
Electrospinning
Scleroproteins
Amino acids
Surface-Active Agents
Disulfides
Extracellular Matrix
Cysteine
glutenin

Keywords

  • 3D electrospinning
  • Adipose-derived mesenchymal stem cells
  • Intrinsic water stability
  • Protein scaffolds
  • Soft tissue engineering
  • Ultrafine fibers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Electrospun ultrafine fibrous wheat glutenin scaffolds with three-dimensionally random organization and water stability for soft tissue engineering. / Xu, Helan; Cai, Shaobo; Sellers, Alexander; Yang, Yiqi.

In: Journal of Biotechnology, Vol. 184, 20.08.2014, p. 179-186.

Research output: Contribution to journalArticle

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