Electrospun nanofiber scaffolds with gradations in fiber organization

Karl Khandalavala, Jiang Jiang, Franklin D. Shuler, Jingwei Xie

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible applications in controlling cell morphology/orientation. Nanofiber organization is controlled with a new fabrication apparatus that enables the gradual decrease of fiber organization in a scaffold. Changing the alignment of fibers is achieved through decreasing deposition time of random electrospun fibers on a uniaxially aligned fiber mat. By covering the collector with a moving barrier/mask, along the same axis as fiber deposition, the organizational structure is easily controlled. For tissue engineering purposes, adipose-derived stem cells can be seeded to these scaffolds. Stem cells undergo morphological changes as a result of their position on the varied organizational structure, and can potentially differentiate into different cell types depending on their locations. Additionally, the graded organization of fibers enhances the biomimicry of nanofiber scaffolds so they more closely resemble the natural orientations of collagen nanofibers at tendon-to-bone insertion site compared to traditional scaffolds. Through nanoencapsulation, the gradated fibers also afford the possibility to construct chemical gradients in fiber scaffolds, and thereby further strengthen their potential applications in fast screening of cell-materials interaction and interfacial tissue regeneration. This technique enables the production of continuous gradient scaffolds, but it also can potentially produce fibers in discrete steps by controlling the movement of the moving barrier/mask in a discrete fashion.

Original languageEnglish (US)
Article numbere52626
JournalJournal of Visualized Experiments
Volume2015
Issue number98
DOIs
StatePublished - Apr 19 2015

Fingerprint

Nanofibers
Scaffolds
Fibers
Masks
Stem Cells
Tissue Engineering
Cell Communication
Tendons
Regeneration
Stem cells
Collagen
Bone and Bones
Tissue regeneration
Tissue engineering
Screening
Bone

Keywords

  • Bioengineering
  • Electrospinning
  • Gradations
  • Issue 98
  • Nanoencapsulation
  • Nanofiber scaffolds
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Electrospun nanofiber scaffolds with gradations in fiber organization. / Khandalavala, Karl; Jiang, Jiang; Shuler, Franklin D.; Xie, Jingwei.

In: Journal of Visualized Experiments, Vol. 2015, No. 98, e52626, 19.04.2015.

Research output: Contribution to journalArticle

Khandalavala, Karl ; Jiang, Jiang ; Shuler, Franklin D. ; Xie, Jingwei. / Electrospun nanofiber scaffolds with gradations in fiber organization. In: Journal of Visualized Experiments. 2015 ; Vol. 2015, No. 98.
@article{8db17e4bb441472dafa1b6e3c4ad2f81,
title = "Electrospun nanofiber scaffolds with gradations in fiber organization",
abstract = "The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible applications in controlling cell morphology/orientation. Nanofiber organization is controlled with a new fabrication apparatus that enables the gradual decrease of fiber organization in a scaffold. Changing the alignment of fibers is achieved through decreasing deposition time of random electrospun fibers on a uniaxially aligned fiber mat. By covering the collector with a moving barrier/mask, along the same axis as fiber deposition, the organizational structure is easily controlled. For tissue engineering purposes, adipose-derived stem cells can be seeded to these scaffolds. Stem cells undergo morphological changes as a result of their position on the varied organizational structure, and can potentially differentiate into different cell types depending on their locations. Additionally, the graded organization of fibers enhances the biomimicry of nanofiber scaffolds so they more closely resemble the natural orientations of collagen nanofibers at tendon-to-bone insertion site compared to traditional scaffolds. Through nanoencapsulation, the gradated fibers also afford the possibility to construct chemical gradients in fiber scaffolds, and thereby further strengthen their potential applications in fast screening of cell-materials interaction and interfacial tissue regeneration. This technique enables the production of continuous gradient scaffolds, but it also can potentially produce fibers in discrete steps by controlling the movement of the moving barrier/mask in a discrete fashion.",
keywords = "Bioengineering, Electrospinning, Gradations, Issue 98, Nanoencapsulation, Nanofiber scaffolds, Stem cells, Tissue engineering",
author = "Karl Khandalavala and Jiang Jiang and Shuler, {Franklin D.} and Jingwei Xie",
year = "2015",
month = "4",
day = "19",
doi = "10.3791/52626",
language = "English (US)",
volume = "2015",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "98",

}

TY - JOUR

T1 - Electrospun nanofiber scaffolds with gradations in fiber organization

AU - Khandalavala, Karl

AU - Jiang, Jiang

AU - Shuler, Franklin D.

AU - Xie, Jingwei

PY - 2015/4/19

Y1 - 2015/4/19

N2 - The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible applications in controlling cell morphology/orientation. Nanofiber organization is controlled with a new fabrication apparatus that enables the gradual decrease of fiber organization in a scaffold. Changing the alignment of fibers is achieved through decreasing deposition time of random electrospun fibers on a uniaxially aligned fiber mat. By covering the collector with a moving barrier/mask, along the same axis as fiber deposition, the organizational structure is easily controlled. For tissue engineering purposes, adipose-derived stem cells can be seeded to these scaffolds. Stem cells undergo morphological changes as a result of their position on the varied organizational structure, and can potentially differentiate into different cell types depending on their locations. Additionally, the graded organization of fibers enhances the biomimicry of nanofiber scaffolds so they more closely resemble the natural orientations of collagen nanofibers at tendon-to-bone insertion site compared to traditional scaffolds. Through nanoencapsulation, the gradated fibers also afford the possibility to construct chemical gradients in fiber scaffolds, and thereby further strengthen their potential applications in fast screening of cell-materials interaction and interfacial tissue regeneration. This technique enables the production of continuous gradient scaffolds, but it also can potentially produce fibers in discrete steps by controlling the movement of the moving barrier/mask in a discrete fashion.

AB - The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible applications in controlling cell morphology/orientation. Nanofiber organization is controlled with a new fabrication apparatus that enables the gradual decrease of fiber organization in a scaffold. Changing the alignment of fibers is achieved through decreasing deposition time of random electrospun fibers on a uniaxially aligned fiber mat. By covering the collector with a moving barrier/mask, along the same axis as fiber deposition, the organizational structure is easily controlled. For tissue engineering purposes, adipose-derived stem cells can be seeded to these scaffolds. Stem cells undergo morphological changes as a result of their position on the varied organizational structure, and can potentially differentiate into different cell types depending on their locations. Additionally, the graded organization of fibers enhances the biomimicry of nanofiber scaffolds so they more closely resemble the natural orientations of collagen nanofibers at tendon-to-bone insertion site compared to traditional scaffolds. Through nanoencapsulation, the gradated fibers also afford the possibility to construct chemical gradients in fiber scaffolds, and thereby further strengthen their potential applications in fast screening of cell-materials interaction and interfacial tissue regeneration. This technique enables the production of continuous gradient scaffolds, but it also can potentially produce fibers in discrete steps by controlling the movement of the moving barrier/mask in a discrete fashion.

KW - Bioengineering

KW - Electrospinning

KW - Gradations

KW - Issue 98

KW - Nanoencapsulation

KW - Nanofiber scaffolds

KW - Stem cells

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84941242220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941242220&partnerID=8YFLogxK

U2 - 10.3791/52626

DO - 10.3791/52626

M3 - Article

C2 - 25938562

AN - SCOPUS:84941242220

VL - 2015

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 98

M1 - e52626

ER -