Electrophoretic studies of the cystic fibrosis ciliary inhibitor and its interaction with immunoglobulin G

Steven D Carson, B. L. Harper, D. R. Barnett

Research output: Contribution to journalArticle

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Abstract

The cystic fibrosis ciliary inhibitor (CFCI) has been partially purified from serum and plasma of cystic fibrosis (CF) homozygotes and heterozygotes, and from media of cultured fibroblasts derived from cystic fibrosis genotypes. Characterization and comparison of fractions containing the CFCI were carried out by polyacrylamide gel electrophoresis. Gel electrophoresis confirmed previous molecular weight estimations of 4,500 to 11,000 for the CFCI and provided an estimate of the number of proteins present in the fractions. Low molecular weight proteins from serum and media were combined with IgG preparations. No specific binding to IgG by the media fraction containing the CFCI could be demonstrated by the techniques employed. There was decreased binding of the low molecular weight serum fraction containing CFC to native IgG molecules from cystic fibrosis patients as compared to IgG from normal individuals. However, IgG from CF individuals demonstrated increased binding of the CFCI containing low molecular weight serum fraction after gel filtration in the presence of guanidinium chloride. This suggests: that very low concentrations of CFCI are present in media fractions; and that native CF IgG cannot bind the low molecular weight CFCI fractions to the same degree as native IgG from normals or CF IgG that has been dissociated from non covalently bound components.

Original languageEnglish (US)
Pages (from-to)209-219
Number of pages11
JournalTexas Reports on Biology and Medicine
Volume34
Issue number1
StatePublished - Jan 1 1976

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Immunoglobulin G
Cystic Fibrosis
Molecular Weight
Serum
ciliary dyskinesia factor
Guanidine
Homozygote
Heterozygote
Gel Chromatography
Electrophoresis
Blood Proteins
Polyacrylamide Gel Electrophoresis
Fibroblasts
Gels
Genotype
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Electrophoretic studies of the cystic fibrosis ciliary inhibitor and its interaction with immunoglobulin G. / Carson, Steven D; Harper, B. L.; Barnett, D. R.

In: Texas Reports on Biology and Medicine, Vol. 34, No. 1, 01.01.1976, p. 209-219.

Research output: Contribution to journalArticle

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