Electroconvulsive seizure increases adult hippocampal angiogenesis in rats

Samuel S. Newton, Matthew J. Girgenti, Emily F. Collier, Ronald S. Duman

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Electroconvulsive seizure has a proven therapeutic application in the treatment of severe depression and treatment-resistant depression. Despite the efficacy of electroconvulsive seizure as a non-chemical antidepressant treatment, the mechanism of action is unclear. Elevation in hippocampal trophic factor expression and concomitant cellular proliferation are thought to play a role in its action. We examined whether the reported induction of angiogenic factors and endothelial cell proliferation leads to an increase in vascular density. Two hippocampal regions, the dentate gyrus and the stratum lacunosum moleculare (SLM), were examined employing a combination of vascular density quantification, angiogenic gene expression analysis and immunohistochemistry. A 6% increase in vascular density was observed in the dentate gyrus but this did not achieve statistical significance. The SLM of the hippocampus exhibited a robust 20-30% increase in vascular density and was accompanied by an increase in expression of inhibitor of differentiation-3. There was also an induction of the angiogenesis markers αVβ3 integrin and Del1. Increases in the vascular density of the SLM could be in response to enhanced metabolic activity in this region. This is supported by the induction of glutamine synthetase and the glutamate transporter GLT1.

Original languageEnglish (US)
Pages (from-to)819-828
Number of pages10
JournalEuropean Journal of Neuroscience
Volume24
Issue number3
DOIs
StatePublished - Aug 1 2006

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Blood Vessels
Seizures
Dentate Gyrus
Cell Proliferation
Treatment-Resistant Depressive Disorder
Amino Acid Transport System X-AG
Glutamate-Ammonia Ligase
Angiogenesis Inducing Agents
Integrins
Antidepressive Agents
Hippocampus
Therapeutics
Endothelial Cells
Immunohistochemistry
Depression
Gene Expression

Keywords

  • Angiogenic factors
  • Antidepressant
  • Gene expression
  • Metabolic demand
  • Stratum lacunosum moleculare
  • Vascular density

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Electroconvulsive seizure increases adult hippocampal angiogenesis in rats. / Newton, Samuel S.; Girgenti, Matthew J.; Collier, Emily F.; Duman, Ronald S.

In: European Journal of Neuroscience, Vol. 24, No. 3, 01.08.2006, p. 819-828.

Research output: Contribution to journalArticle

Newton, Samuel S. ; Girgenti, Matthew J. ; Collier, Emily F. ; Duman, Ronald S. / Electroconvulsive seizure increases adult hippocampal angiogenesis in rats. In: European Journal of Neuroscience. 2006 ; Vol. 24, No. 3. pp. 819-828.
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