Electrical stimulation of thalamic Nucleus Submedius inhibits responses of spinal dorsal horn neurons to colorectal distension in the rat

Shou Wei Yang, Kenneth A. Follett

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In 78 halothane-anesthetized rats, we characterized the responses of single neurons in the dorsal horn of L6-S1 spinal segments to a noxious visceral stimulus (colorectal balloon distension, CRD), and studied the effects of focal electrical stimulation of Nucleus Submedius (Sm) on these responses using standard extracellular microelectrode recording techniques. A total of 102 neurons were isolated on the basis of spontaneous activity. Eighty (78%) responded to CRD, of which 70% had excitatory and 30% had inhibitory responses. Neurons showed graded responses to graded CRD pressures (20-100mmHg), with maximum excitation or inhibition occurring at 100mmHg. Responses to noxious (pinch, heat) and innocuous (brush, tap) cutaneous stimuli were studied in 73 of the spinal dorsal horn neurons isolated. Fifty-seven (78%) of these neurons (46 CRD-responsive and 11 CRD-nonresponsive) had cutaneous receptive fields, of which 35 (61%) were small and ipsilateral, 14 (25%) were large and ipsilateral, 7 (12%) were large or small and bilateral, and 1 (2%) was small and contralateral. Sixty-one percent of these neurons responded to both noxious and innocuous cutaneous stimulation, 35% responded only to noxious stimulation, and 4% responded only to innocuous stimulation. Electrical stimulation (50-300μA) of the contralateral Sm produced intensity-dependent attenuation of the CRD-evoked activities of most neurons (18/28 of CRD-excited and 7/12 of CRD-inhibited) tested. Sm stimulation produced facilitation of CRD responses of only one neuron (CRD-inhibited). Sm stimulation had no effects on spontaneous activity. These data indicate that Sm may be involved in the descending inhibitory modulation of visceral nociception at the spinal level.

Original languageEnglish (US)
Pages (from-to)413-420
Number of pages8
JournalBrain Research Bulletin
Volume59
Issue number6
DOIs
StatePublished - Feb 15 2003

Fingerprint

Posterior Horn Cells
Thalamic Nuclei
Electric Stimulation
Neurons
Skin
Nociception
Microelectrodes
Halothane
Hot Temperature
Pressure

Keywords

  • Analgesia
  • Descending modulation
  • Electrophysiological recording
  • Medial thalamus
  • Spinal cord
  • Visceral pain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{4a544295104247ffb435d0649b38436f,
title = "Electrical stimulation of thalamic Nucleus Submedius inhibits responses of spinal dorsal horn neurons to colorectal distension in the rat",
abstract = "In 78 halothane-anesthetized rats, we characterized the responses of single neurons in the dorsal horn of L6-S1 spinal segments to a noxious visceral stimulus (colorectal balloon distension, CRD), and studied the effects of focal electrical stimulation of Nucleus Submedius (Sm) on these responses using standard extracellular microelectrode recording techniques. A total of 102 neurons were isolated on the basis of spontaneous activity. Eighty (78{\%}) responded to CRD, of which 70{\%} had excitatory and 30{\%} had inhibitory responses. Neurons showed graded responses to graded CRD pressures (20-100mmHg), with maximum excitation or inhibition occurring at 100mmHg. Responses to noxious (pinch, heat) and innocuous (brush, tap) cutaneous stimuli were studied in 73 of the spinal dorsal horn neurons isolated. Fifty-seven (78{\%}) of these neurons (46 CRD-responsive and 11 CRD-nonresponsive) had cutaneous receptive fields, of which 35 (61{\%}) were small and ipsilateral, 14 (25{\%}) were large and ipsilateral, 7 (12{\%}) were large or small and bilateral, and 1 (2{\%}) was small and contralateral. Sixty-one percent of these neurons responded to both noxious and innocuous cutaneous stimulation, 35{\%} responded only to noxious stimulation, and 4{\%} responded only to innocuous stimulation. Electrical stimulation (50-300μA) of the contralateral Sm produced intensity-dependent attenuation of the CRD-evoked activities of most neurons (18/28 of CRD-excited and 7/12 of CRD-inhibited) tested. Sm stimulation produced facilitation of CRD responses of only one neuron (CRD-inhibited). Sm stimulation had no effects on spontaneous activity. These data indicate that Sm may be involved in the descending inhibitory modulation of visceral nociception at the spinal level.",
keywords = "Analgesia, Descending modulation, Electrophysiological recording, Medial thalamus, Spinal cord, Visceral pain",
author = "Yang, {Shou Wei} and Follett, {Kenneth A.}",
year = "2003",
month = "2",
day = "15",
doi = "10.1016/S0361-9230(02)00945-0",
language = "English (US)",
volume = "59",
pages = "413--420",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Electrical stimulation of thalamic Nucleus Submedius inhibits responses of spinal dorsal horn neurons to colorectal distension in the rat

AU - Yang, Shou Wei

AU - Follett, Kenneth A.

PY - 2003/2/15

Y1 - 2003/2/15

N2 - In 78 halothane-anesthetized rats, we characterized the responses of single neurons in the dorsal horn of L6-S1 spinal segments to a noxious visceral stimulus (colorectal balloon distension, CRD), and studied the effects of focal electrical stimulation of Nucleus Submedius (Sm) on these responses using standard extracellular microelectrode recording techniques. A total of 102 neurons were isolated on the basis of spontaneous activity. Eighty (78%) responded to CRD, of which 70% had excitatory and 30% had inhibitory responses. Neurons showed graded responses to graded CRD pressures (20-100mmHg), with maximum excitation or inhibition occurring at 100mmHg. Responses to noxious (pinch, heat) and innocuous (brush, tap) cutaneous stimuli were studied in 73 of the spinal dorsal horn neurons isolated. Fifty-seven (78%) of these neurons (46 CRD-responsive and 11 CRD-nonresponsive) had cutaneous receptive fields, of which 35 (61%) were small and ipsilateral, 14 (25%) were large and ipsilateral, 7 (12%) were large or small and bilateral, and 1 (2%) was small and contralateral. Sixty-one percent of these neurons responded to both noxious and innocuous cutaneous stimulation, 35% responded only to noxious stimulation, and 4% responded only to innocuous stimulation. Electrical stimulation (50-300μA) of the contralateral Sm produced intensity-dependent attenuation of the CRD-evoked activities of most neurons (18/28 of CRD-excited and 7/12 of CRD-inhibited) tested. Sm stimulation produced facilitation of CRD responses of only one neuron (CRD-inhibited). Sm stimulation had no effects on spontaneous activity. These data indicate that Sm may be involved in the descending inhibitory modulation of visceral nociception at the spinal level.

AB - In 78 halothane-anesthetized rats, we characterized the responses of single neurons in the dorsal horn of L6-S1 spinal segments to a noxious visceral stimulus (colorectal balloon distension, CRD), and studied the effects of focal electrical stimulation of Nucleus Submedius (Sm) on these responses using standard extracellular microelectrode recording techniques. A total of 102 neurons were isolated on the basis of spontaneous activity. Eighty (78%) responded to CRD, of which 70% had excitatory and 30% had inhibitory responses. Neurons showed graded responses to graded CRD pressures (20-100mmHg), with maximum excitation or inhibition occurring at 100mmHg. Responses to noxious (pinch, heat) and innocuous (brush, tap) cutaneous stimuli were studied in 73 of the spinal dorsal horn neurons isolated. Fifty-seven (78%) of these neurons (46 CRD-responsive and 11 CRD-nonresponsive) had cutaneous receptive fields, of which 35 (61%) were small and ipsilateral, 14 (25%) were large and ipsilateral, 7 (12%) were large or small and bilateral, and 1 (2%) was small and contralateral. Sixty-one percent of these neurons responded to both noxious and innocuous cutaneous stimulation, 35% responded only to noxious stimulation, and 4% responded only to innocuous stimulation. Electrical stimulation (50-300μA) of the contralateral Sm produced intensity-dependent attenuation of the CRD-evoked activities of most neurons (18/28 of CRD-excited and 7/12 of CRD-inhibited) tested. Sm stimulation produced facilitation of CRD responses of only one neuron (CRD-inhibited). Sm stimulation had no effects on spontaneous activity. These data indicate that Sm may be involved in the descending inhibitory modulation of visceral nociception at the spinal level.

KW - Analgesia

KW - Descending modulation

KW - Electrophysiological recording

KW - Medial thalamus

KW - Spinal cord

KW - Visceral pain

UR - http://www.scopus.com/inward/record.url?scp=0037441574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037441574&partnerID=8YFLogxK

U2 - 10.1016/S0361-9230(02)00945-0

DO - 10.1016/S0361-9230(02)00945-0

M3 - Article

C2 - 12576136

AN - SCOPUS:0037441574

VL - 59

SP - 413

EP - 420

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 6

ER -