Egg ovotransferrin-derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats

Kaustav Majumder, Guanxiang Liang, Yanhong Chen, Leluo Guan, Sandra T. Davidge, Jianping Wu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Scope: Egg ovotransferrin-derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide-mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. Methods and results: Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW-treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW-treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE-2, ABCB-1, IRF-8, and CDH-1 while significantly decreased the expression ICAM-1 and VCAM-1 in mesenteric arteries. Conclusion: Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.

Original languageEnglish (US)
Pages (from-to)1735-1744
Number of pages10
JournalMolecular Nutrition and Food Research
Volume59
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Conalbumin
mesenteric arteries
Mesenteric Arteries
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
Inbred SHR Rats
Ovum
peptides
Gene Expression
gene expression
Peptides
rats
kidneys
Kidney
Genes
antihypertensive effect
genes
Vascular Cell Adhesion Molecule-1
vasodilation
Gene Expression Profiling

Keywords

  • ACE-2
  • Bioactive peptides
  • Hypertension
  • RNAseq
  • SHR

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Egg ovotransferrin-derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats. / Majumder, Kaustav; Liang, Guanxiang; Chen, Yanhong; Guan, Leluo; Davidge, Sandra T.; Wu, Jianping.

In: Molecular Nutrition and Food Research, Vol. 59, No. 9, 01.09.2015, p. 1735-1744.

Research output: Contribution to journalArticle

@article{c11115f857e94a0fa2100754637a8539,
title = "Egg ovotransferrin-derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats",
abstract = "Scope: Egg ovotransferrin-derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide-mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. Methods and results: Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW-treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW-treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE-2, ABCB-1, IRF-8, and CDH-1 while significantly decreased the expression ICAM-1 and VCAM-1 in mesenteric arteries. Conclusion: Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.",
keywords = "ACE-2, Bioactive peptides, Hypertension, RNAseq, SHR",
author = "Kaustav Majumder and Guanxiang Liang and Yanhong Chen and Leluo Guan and Davidge, {Sandra T.} and Jianping Wu",
year = "2015",
month = "9",
day = "1",
doi = "10.1002/mnfr.201500050",
language = "English (US)",
volume = "59",
pages = "1735--1744",
journal = "Molecular Nutrition and Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Egg ovotransferrin-derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats

AU - Majumder, Kaustav

AU - Liang, Guanxiang

AU - Chen, Yanhong

AU - Guan, Leluo

AU - Davidge, Sandra T.

AU - Wu, Jianping

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Scope: Egg ovotransferrin-derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide-mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. Methods and results: Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW-treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW-treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE-2, ABCB-1, IRF-8, and CDH-1 while significantly decreased the expression ICAM-1 and VCAM-1 in mesenteric arteries. Conclusion: Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.

AB - Scope: Egg ovotransferrin-derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide-mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. Methods and results: Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW-treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW-treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE-2, ABCB-1, IRF-8, and CDH-1 while significantly decreased the expression ICAM-1 and VCAM-1 in mesenteric arteries. Conclusion: Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.

KW - ACE-2

KW - Bioactive peptides

KW - Hypertension

KW - RNAseq

KW - SHR

UR - http://www.scopus.com/inward/record.url?scp=84942826619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84942826619&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201500050

DO - 10.1002/mnfr.201500050

M3 - Article

VL - 59

SP - 1735

EP - 1744

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 9

ER -