Egg intake during carbohydrate restriction alters peripheral blood mononuclear cell inflammation and cholesterol homeostasis in metabolic syndrome

Catherine J. Andersen, Ji Young Lee, Christopher N. Blesso, Timothy P. Carr, Maria Luz Fernandez

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Egg yolk contains bioactive components that improve plasma inflammatory markers and HDL profiles in metabolic syndrome (MetS) under carbohydrate restriction. We further sought to determine whether egg yolk intake affects peripheral blood mononuclear cell (PBMC) inflammation and cholesterol homeostasis in MetS, as HDL and its associated lipid transporter ATP-binding cassette transporter A1 (ABCA1) reduce the inflammatory potential of leukocytes through modulation of cellular cholesterol content and distribution. Thirty-seven men and women classified with MetS consumed a moderate carbohydrate-restricted diet (25%-30% of energy) for 12 weeks, in addition to consuming either three whole eggs per day (EGG) or the equivalent amount of yolk-free egg substitute (SUB). Interestingly, lipopolysaccharide-induced PBMC IL-1β and TNFα secretion increased from baseline to week 12 in the SUB group only, despite increases in PBMC toll-like receptor 4 (TLR4) mRNA expression in the EGG group. Compared to baseline, ABCA1 and 3-hydroxy-3-methyl-glutaryl (HMG)-CoA reductase mRNA expression increased by week 12 in the EGG group only, whereas changes in PBMC total cholesterol positively correlated with changes in lipid raft content. Together, these findings suggest that intake of whole eggs during carbohydrate restriction alters PBMC inflammation and cholesterol homeostasis in MetS.

Original languageEnglish (US)
Pages (from-to)2650-2667
Number of pages18
JournalNutrients
Volume6
Issue number7
DOIs
StatePublished - Jul 18 2014

Fingerprint

carbohydrate intake
metabolic syndrome
mononuclear leukocytes
Ovum
Blood Cells
homeostasis
Homeostasis
inflammation
Cholesterol
Carbohydrates
cholesterol
Egg Yolk
Inflammation
ABC transporters
ATP-Binding Cassette Transporters
carbohydrates
egg yolk
Eggs
egg substitutes
Carbohydrate-Restricted Diet

Keywords

  • ABCA1
  • Cholesterol
  • Eggs
  • Inflammation
  • Metabolic syndrome
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Egg intake during carbohydrate restriction alters peripheral blood mononuclear cell inflammation and cholesterol homeostasis in metabolic syndrome. / Andersen, Catherine J.; Lee, Ji Young; Blesso, Christopher N.; Carr, Timothy P.; Fernandez, Maria Luz.

In: Nutrients, Vol. 6, No. 7, 18.07.2014, p. 2650-2667.

Research output: Contribution to journalArticle

Andersen, Catherine J. ; Lee, Ji Young ; Blesso, Christopher N. ; Carr, Timothy P. ; Fernandez, Maria Luz. / Egg intake during carbohydrate restriction alters peripheral blood mononuclear cell inflammation and cholesterol homeostasis in metabolic syndrome. In: Nutrients. 2014 ; Vol. 6, No. 7. pp. 2650-2667.
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