Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats

Kaustav Majumder, Subhadeep Chakrabarti, Jude S. Morton, Sareh Panahi, Susan Kaufman, Sandra T. Davidge, Jianping Wu

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Background: There is a growing interest in using functional food components as therapy for cardiovascular diseases such as hypertension. We have previously characterized a tri-peptide IRW (Ile-Arg-Trp) from egg white protein ovotransferrin; this peptide showed anti-inflammatory, anti-oxidant and angiotensin converting enzyme (ACE) inhibitor properties in vitro. Given the pathogenic roles played by angiotensin, oxidative stress and inflammation in the spontaneously hypertensive rat (SHR), we tested the therapeutic potential of IRW in this well-established model of hypertension. Methods and Results: 16-17 week old male SHRs were orally administered IRW at either a low dose (3 mg/Kg BW) or a high dose (15 mg/Kg BW) daily for 18 days. Blood pressure (BP) and heart rate were measured by telemetry. Animals were sacrificed at the end of the treatment for vascular function studies and measuring markers of inflammation. IRW treatment attenuated mean BP by ∼10 mmHg and ∼40 mmHg at the low- and high-dose groups respectively compared to untreated SHRs. Heart rate was not affected. Reduction in BP was accompanied by the restoration of diurnal variations in BP, preservation of nitric oxide dependent vasorelaxation, as well as reduction of plasma angiotensin II, other inflammatory markers and tissue fibrosis. Conclusion: Our results demonstrate anti-hypertensive effects of IRW in vivo likely mediated through ACE inhibition, endothelial nitric oxide synthase and anti-inflammatory properties.

Original languageEnglish (US)
Article numbere82829
JournalPloS one
Volume8
Issue number11
DOIs
StatePublished - Nov 29 2013

Fingerprint

antihypertensive effect
Blood pressure
Inbred SHR Rats
Antihypertensive Agents
blood pressure
Ovum
Rats
peptides
Blood Pressure
Peptides
rats
dosage
hypertension
heart rate
Anti-Inflammatory Agents
inflammation
Heart Rate
Conalbumin
Blood Preservation
Hypertension

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Majumder, K., Chakrabarti, S., Morton, J. S., Panahi, S., Kaufman, S., Davidge, S. T., & Wu, J. (2013). Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats. PloS one, 8(11), [e82829]. https://doi.org/10.1371/journal.pone.0082829

Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats. / Majumder, Kaustav; Chakrabarti, Subhadeep; Morton, Jude S.; Panahi, Sareh; Kaufman, Susan; Davidge, Sandra T.; Wu, Jianping.

In: PloS one, Vol. 8, No. 11, e82829, 29.11.2013.

Research output: Contribution to journalArticle

Majumder, K, Chakrabarti, S, Morton, JS, Panahi, S, Kaufman, S, Davidge, ST & Wu, J 2013, 'Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats', PloS one, vol. 8, no. 11, e82829. https://doi.org/10.1371/journal.pone.0082829
Majumder, Kaustav ; Chakrabarti, Subhadeep ; Morton, Jude S. ; Panahi, Sareh ; Kaufman, Susan ; Davidge, Sandra T. ; Wu, Jianping. / Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats. In: PloS one. 2013 ; Vol. 8, No. 11.
@article{eb41e5ab5ec0478c9a19705af85fa2e1,
title = "Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats",
abstract = "Background: There is a growing interest in using functional food components as therapy for cardiovascular diseases such as hypertension. We have previously characterized a tri-peptide IRW (Ile-Arg-Trp) from egg white protein ovotransferrin; this peptide showed anti-inflammatory, anti-oxidant and angiotensin converting enzyme (ACE) inhibitor properties in vitro. Given the pathogenic roles played by angiotensin, oxidative stress and inflammation in the spontaneously hypertensive rat (SHR), we tested the therapeutic potential of IRW in this well-established model of hypertension. Methods and Results: 16-17 week old male SHRs were orally administered IRW at either a low dose (3 mg/Kg BW) or a high dose (15 mg/Kg BW) daily for 18 days. Blood pressure (BP) and heart rate were measured by telemetry. Animals were sacrificed at the end of the treatment for vascular function studies and measuring markers of inflammation. IRW treatment attenuated mean BP by ∼10 mmHg and ∼40 mmHg at the low- and high-dose groups respectively compared to untreated SHRs. Heart rate was not affected. Reduction in BP was accompanied by the restoration of diurnal variations in BP, preservation of nitric oxide dependent vasorelaxation, as well as reduction of plasma angiotensin II, other inflammatory markers and tissue fibrosis. Conclusion: Our results demonstrate anti-hypertensive effects of IRW in vivo likely mediated through ACE inhibition, endothelial nitric oxide synthase and anti-inflammatory properties.",
author = "Kaustav Majumder and Subhadeep Chakrabarti and Morton, {Jude S.} and Sareh Panahi and Susan Kaufman and Davidge, {Sandra T.} and Jianping Wu",
year = "2013",
month = "11",
day = "29",
doi = "10.1371/journal.pone.0082829",
language = "English (US)",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Egg-derived tri-peptide IRW exerts antihypertensive effects in spontaneously hypertensive rats

AU - Majumder, Kaustav

AU - Chakrabarti, Subhadeep

AU - Morton, Jude S.

AU - Panahi, Sareh

AU - Kaufman, Susan

AU - Davidge, Sandra T.

AU - Wu, Jianping

PY - 2013/11/29

Y1 - 2013/11/29

N2 - Background: There is a growing interest in using functional food components as therapy for cardiovascular diseases such as hypertension. We have previously characterized a tri-peptide IRW (Ile-Arg-Trp) from egg white protein ovotransferrin; this peptide showed anti-inflammatory, anti-oxidant and angiotensin converting enzyme (ACE) inhibitor properties in vitro. Given the pathogenic roles played by angiotensin, oxidative stress and inflammation in the spontaneously hypertensive rat (SHR), we tested the therapeutic potential of IRW in this well-established model of hypertension. Methods and Results: 16-17 week old male SHRs were orally administered IRW at either a low dose (3 mg/Kg BW) or a high dose (15 mg/Kg BW) daily for 18 days. Blood pressure (BP) and heart rate were measured by telemetry. Animals were sacrificed at the end of the treatment for vascular function studies and measuring markers of inflammation. IRW treatment attenuated mean BP by ∼10 mmHg and ∼40 mmHg at the low- and high-dose groups respectively compared to untreated SHRs. Heart rate was not affected. Reduction in BP was accompanied by the restoration of diurnal variations in BP, preservation of nitric oxide dependent vasorelaxation, as well as reduction of plasma angiotensin II, other inflammatory markers and tissue fibrosis. Conclusion: Our results demonstrate anti-hypertensive effects of IRW in vivo likely mediated through ACE inhibition, endothelial nitric oxide synthase and anti-inflammatory properties.

AB - Background: There is a growing interest in using functional food components as therapy for cardiovascular diseases such as hypertension. We have previously characterized a tri-peptide IRW (Ile-Arg-Trp) from egg white protein ovotransferrin; this peptide showed anti-inflammatory, anti-oxidant and angiotensin converting enzyme (ACE) inhibitor properties in vitro. Given the pathogenic roles played by angiotensin, oxidative stress and inflammation in the spontaneously hypertensive rat (SHR), we tested the therapeutic potential of IRW in this well-established model of hypertension. Methods and Results: 16-17 week old male SHRs were orally administered IRW at either a low dose (3 mg/Kg BW) or a high dose (15 mg/Kg BW) daily for 18 days. Blood pressure (BP) and heart rate were measured by telemetry. Animals were sacrificed at the end of the treatment for vascular function studies and measuring markers of inflammation. IRW treatment attenuated mean BP by ∼10 mmHg and ∼40 mmHg at the low- and high-dose groups respectively compared to untreated SHRs. Heart rate was not affected. Reduction in BP was accompanied by the restoration of diurnal variations in BP, preservation of nitric oxide dependent vasorelaxation, as well as reduction of plasma angiotensin II, other inflammatory markers and tissue fibrosis. Conclusion: Our results demonstrate anti-hypertensive effects of IRW in vivo likely mediated through ACE inhibition, endothelial nitric oxide synthase and anti-inflammatory properties.

UR - http://www.scopus.com/inward/record.url?scp=84896691651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896691651&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0082829

DO - 10.1371/journal.pone.0082829

M3 - Article

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e82829

ER -