Egg-derived peptide IRW inhibits TNF-α-induced inflammatory response and oxidative stress in endothelial cells

Wuyang Huang, Subhadeep Chakrabarti, Kaustav Majumder, Yanyan Jiang, Sandra T. Davidge, Jianping Wu

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Oxidative stress and vascular inflammatory response are key mediators of endothelial dysfunction that leads to cardiovascular diseases. A novel peptide, IRW, was previously characterized from egg protein with angiotensin converting enzyme inhibitory activity. The purpose of the study was to investigate the effects and molecular mechanisms of IRW on regulating inflammatory response in endothelial cells. The results showed that tumor necrosis factor-R (TNF-α) significantly increased the protein levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic protein-1 (MCP-1), whereas pretreatment with IRW inhibited TNF-α-induced increases of ICAM-1, VCAM-1, and MCP-1 production in a concentration-dependent manner. IRW also reduced the levels of superoxide ions in the presence and absence of TNF-α. These results indicate the potential role of IRW in preventing cardiovascular disease as a functional food ingredient or nutraceutical.

Original languageEnglish (US)
Pages (from-to)10840-10846
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number20
DOIs
StatePublished - Oct 27 2010

Fingerprint

Oxidative stress
tumor necrosis factors
Endothelial cells
blood vessels
endothelial cells
Ovum
Oxidative Stress
oxidative stress
Endothelial Cells
Vascular Cell Adhesion Molecule-1
Tumor Necrosis Factor-alpha
Chemokine CCL2
inflammation
peptides
Intercellular Adhesion Molecule-1
cell adhesion
functional foods
Peptides
cardiovascular diseases
adhesion

Keywords

  • Anti-inflammatory
  • Antioxidant
  • Egg peptide IRW
  • Endothelial cells
  • Tumor necrosis factor-α (TNF-α)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Egg-derived peptide IRW inhibits TNF-α-induced inflammatory response and oxidative stress in endothelial cells. / Huang, Wuyang; Chakrabarti, Subhadeep; Majumder, Kaustav; Jiang, Yanyan; Davidge, Sandra T.; Wu, Jianping.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 20, 27.10.2010, p. 10840-10846.

Research output: Contribution to journalArticle

Huang, Wuyang ; Chakrabarti, Subhadeep ; Majumder, Kaustav ; Jiang, Yanyan ; Davidge, Sandra T. ; Wu, Jianping. / Egg-derived peptide IRW inhibits TNF-α-induced inflammatory response and oxidative stress in endothelial cells. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 20. pp. 10840-10846.
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AU - Majumder, Kaustav

AU - Jiang, Yanyan

AU - Davidge, Sandra T.

AU - Wu, Jianping

PY - 2010/10/27

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N2 - Oxidative stress and vascular inflammatory response are key mediators of endothelial dysfunction that leads to cardiovascular diseases. A novel peptide, IRW, was previously characterized from egg protein with angiotensin converting enzyme inhibitory activity. The purpose of the study was to investigate the effects and molecular mechanisms of IRW on regulating inflammatory response in endothelial cells. The results showed that tumor necrosis factor-R (TNF-α) significantly increased the protein levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic protein-1 (MCP-1), whereas pretreatment with IRW inhibited TNF-α-induced increases of ICAM-1, VCAM-1, and MCP-1 production in a concentration-dependent manner. IRW also reduced the levels of superoxide ions in the presence and absence of TNF-α. These results indicate the potential role of IRW in preventing cardiovascular disease as a functional food ingredient or nutraceutical.

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