Efficient retroviral transfer of a mouse c-myc construct into HL60

Gábor Szabó, Gösta Winberg, Marie Henriksson, George Klein, János Sümegi

Research output: Contribution to journalArticle

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We introduced an LTR-driven mouse c-myc second and third exon, Tn5Neo gene construct into the inducible human leukemia line HL60 using an amphotropic retroviral vector system. Over 90 % of the cells became neo-resistant and the transfected myc gene was transcribed in several neomycin resistant clones. Making use of the simultaneous presence of the different myc genes in the same cell, we compared expression of the corresponding mRNAs after differentiation and their decay mechanisms.

Original languageEnglish (US)
Pages (from-to)321-327
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Aug 30 1989


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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