Efficient infection of Nicotiana benthamiana by Tomato bushy stunt virus is facilitated by the coat protein and maintained by p19 through suppression of gene silencing

Feng Qu, T. Jack Morris

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in some hosts in the absence of coat protein (CP). TBSV also encodes another protein (p19) that is not required for systemic movement but functions as a symptom determinant in Nicotiana benthamiana. Here, the role of both CP and p19 in the systemic spread has been reevaluated by utilizing transgenic N. benthamiana plants expressing the movement protein (MP) of Red clover necrotic mosaic virus and chimeric TBSV mutants that express CP of Turnip crinkle virus. Through careful examination of the infection phenotype of a series of mutants with changes in the CP and p19 genes, we demonstrate that both of these genes are required for efficient systemic invasion of TBSV in N. benthamiana. The CP likely enables efficient viral unloading from the vascular system in the form of assembled virions, whereas p19 enhances systemic infection by suppressing the virus-induced gene silencing.

Original languageEnglish (US)
Pages (from-to)193-202
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume15
Issue number3
DOIs
StatePublished - Jan 1 2002

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Tomato bushy stunt virus
Tombusvirus
Nicotiana benthamiana
Capsid Proteins
Gene Silencing
gene silencing
coat proteins
Viruses
Tobacco
Genes
Infection
infection
Red clover necrotic mosaic virus
Tombusviridae
Carmovirus
Turnip crinkle virus
Plant Viruses
mutants
plant vascular system
RNA Viruses

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

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abstract = "Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in some hosts in the absence of coat protein (CP). TBSV also encodes another protein (p19) that is not required for systemic movement but functions as a symptom determinant in Nicotiana benthamiana. Here, the role of both CP and p19 in the systemic spread has been reevaluated by utilizing transgenic N. benthamiana plants expressing the movement protein (MP) of Red clover necrotic mosaic virus and chimeric TBSV mutants that express CP of Turnip crinkle virus. Through careful examination of the infection phenotype of a series of mutants with changes in the CP and p19 genes, we demonstrate that both of these genes are required for efficient systemic invasion of TBSV in N. benthamiana. The CP likely enables efficient viral unloading from the vascular system in the form of assembled virions, whereas p19 enhances systemic infection by suppressing the virus-induced gene silencing.",
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