Efficiency of neutralizing antibodies targeting the CD4-binding site: Influence of conformational masking by the V2 loop in R5-tropic clade C simian-human immunodeficiency virus

Jennifer D. Watkins, Juan Diaz-Rodriguez, Nagadenahalli B. Siddappa, Davide Corti, Ruth M. Ruprecht

Research output: Contribution to journalComment/debate

10 Scopus citations

Abstract

In R5-tropic clade C simian-human immunodeficiency viruses (SHIV-Cs), we identified a 3-asparagine (3N) deletion mutation in the V2 loop stem of gp120 as the major determinant of neutralization escape of the anti-CD4-binding site (anti-CD4-bs) neutralizing monoclonal antibody (nMAb) b12. However, the more potent anti-CD4-bs nMAbs VRC01 and VRC03 were not sensitive to this mutation. Using isogenic tier 1 or tier 2 proviruses differing only in the 3N mutation, we showed that this mutation might result in selective conformational b12 epitope masking. Therefore, human immunodeficiency virus (HIV) Env immunogens targeting the CD4-bs and designed to neutralize tier 2 viruses should take conformational masking by the V2 loop into account.

Original languageEnglish (US)
Pages (from-to)12811-12814
Number of pages4
JournalJournal of virology
Volume85
Issue number23
DOIs
StatePublished - Dec 1 2011

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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